Prostacyclin, prostaglandin F2 alpha and progesterone production by bovine luteal cells during the estrous cycle

• Published in: Biology of reproduction Vol. 29; no. 5; pp. 1063 - 1068
• Main Authors: MILVAE, R. A, HANSEL, W
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01-12-1983
• Subjects: 6-Ketoprostaglandin F1 alpha - analysis; Animals; Arachidonic Acid; Arachidonic Acids - pharmacology; Biological and medical sciences; Cattle; Corpus Luteum - drug effects; Corpus Luteum - metabolism; Dinoprost; Endocrine pancreas; Epoprostenol - biosynthesis; Estrus; Female; Fundamental and applied biological sciences. Psychology; Hormones. Régulation; Indomethacin - pharmacology; Pregnancy; Progesterone - biosynthesis; Prostaglandins F - biosynthesis; Vertebrates: endocrinology; Vertebrata; Mammalia; Prostaglandin I2; Arachidonic acid derivatives; Corpus luteum; Artiodactyla; Beef; Estrous cycle; Progesterone; Ungulata; Prostaglandin F2α
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod29.5.1063

Corpora lutea (CL) were collected from Holstein heifers on Days 5, 10, 15 and 18 (5/day) of the estrous cycle. Dispersed luteal
cell preparations were made and 10(6) viable luteal cells were incubated with bovine luteinizing hormone (LH) and different
amounts of arachidonic acid in the presence and absence of the prostaglandin (PG) synthetase inhibitor indomethacin. The concentrations
of progesterone, PGF2 alpha and 6-keto-PGF1 alpha, the stable inactive metabolite of prostacyclin (PGI2), were measured. Day
5 CL had the greatest initial content of 6-keto-PGF1 alpha (1.01 +/- 0.16 ng/10(6) cells), and synthesized more 6-keto-PGF1
alpha (2.55 +/- 0.43) than CL collected on Days 10 (0.57 +/- 0.11), 15 (0.08 +/- 0.05) and 18 (0.19 +/- 0.03) during a 2-h
incubation period. Arachidonic acid stimulated the production of 6-keto-PGF1 alpha by Days 10, 15 and 18 luteal tissue. PGF2
alpha was produced at a greater rate on Day 5 (0.69 +/- 0.17 ng/10(6) cells) than on Days 10 (0.06 +/- 0.01), 15 (0.04 +/-
0.02) and 18 (0.08 +/- 0.01). Arachidonic acid stimulated and indomethacin inhibited the production of PGF2 alpha, in most
cases. The initial content of 6-keto-PGF1 alpha was higher than that of PGF2 alpha on all days of the cycle and more 6-keto-PGF1
alpha was synthesized in response to arachidonic acid addition. The ratio of 6-keto-PGF1 alpha content to PGF2 alpha content
was 4.39, 2.30, 1.25 and 1.13 on Days 5, 10, 15 and 18, respectively.