Comparative evaluation of a commercial microdilution method and a conventional disk diffusion method for determination of antibiograms of gram-positive cocci

A commercial broth microdilution system for testing the antimicrobial susceptibility of gram-positive cocci was compared with the standardized disk agar-diffusion method by testing 254 clinical strains of staphylococci and streptococci using both methods. A total of 2,794 parallel determinations wer...

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Bibliographic Details
Published in:	American journal of veterinary research Vol. 46; no. 1; p. 294
Main Authors:	Newman, S S, Roy, A F, Luther, D G, Hoskins, J D, Cox, H U, Kearney, M T
Format:	Journal Article
Language:	English
Published:	United States 01-01-1985
Subjects:	Anti-Bacterial Agents - pharmacology Immunodiffusion Indicator Dilution Techniques Microbial Sensitivity Tests - methods Staphylococcus - drug effects Streptococcus - drug effects
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Summary:	A commercial broth microdilution system for testing the antimicrobial susceptibility of gram-positive cocci was compared with the standardized disk agar-diffusion method by testing 254 clinical strains of staphylococci and streptococci using both methods. A total of 2,794 parallel determinations were made with 92.3% complete agreement between the 2 methods; of the discrepancies encountered, 3.0% were minor, 2.5% were major, and 2.1% were very major. The results indicate that the commercial microdilution system may provide a reliable quantitative method for antimicrobial susceptibility testing of clinical isolates from animals.
ISSN:	0002-9645