Neuroglian is expressed on cells destined to form the prothoracic glands of Manduca embryos as they segregate from surrounding cells and rearrange during morphogenesis

• Published in: Developmental biology Vol. 181; no. 1; pp. 1 - 13
• Main Authors: Chen, C.L, Lampe, D.J, Robertson, H.M, Nardi, J.B
• Format: Journal Article
• Language: English
• Published: United States 1997
• Subjects: Amino Acid Sequence; animal breeding; animal genetics; animal physiology; Animals; arthropod pests; arthropods; Cell Adhesion Molecules, Neuronal - biosynthesis; Cell Adhesion Molecules, Neuronal - genetics; Cell Differentiation; DNA, Complementary - genetics; Drosophila - genetics; Drosophila Proteins; Embryo, Nonmammalian - anatomy & histology; Embryo, Nonmammalian - metabolism; Endocrine Glands - embryology; Endocrine Glands - metabolism; Endocrine Glands - ultrastructure; entomology; Epithelial Cells; Epithelium - metabolism; genbank/u50719; Gene Expression Regulation, Developmental; Genes, Insect; In Situ Hybridization; Insect Proteins - biosynthesis; Insect Proteins - genetics; Intercellular Junctions - metabolism; Larva - metabolism; Manduca - embryology; Manduca - genetics; Manduca - growth & development; Manduca - metabolism; Mice; Molecular Sequence Data; Morphogenesis; plant health; plant pests; Sequence Alignment; Sequence Homology, Amino Acid; Species Specificity; Wings, Animal - metabolism
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1006/dbio.1996.8437

A cell surface protein (3B11) is differentially expressed in the embryonic labial segment of Manduca as two circular monolayers of epithelial cells invaginate and segregate from surrounding epithelial cells. The cells that invaginate and preferentially express 3B11 represent the presumptive prothoracic glands. These cells continue to express protein 3B11 as they rearrange to form first a three-dimensional aggregate and later anastomosing filaments of cells. In the differentiated prothoracic gland, expression of 3B11 is restricted to sites of cell-cell contact. Cloning and sequencing of the cDNA for protein 3B11 revealed that this protein is the Manduca counterpart of Drosophila neuroglian and mouse L1. These surface proteins are known to function as adhesion/recognition molecules during development. Manduca neuroglian shares 58 and 31% identity respectively with the Drosophila and mouse proteins and has a cytoplasmic domain of over 100 amino acids.