Evidence for the replication of bovine leukemia virus in the B lymphocytes

Bovine peripheral blood lymphocytes from a cow with persistent lymphocytosis were separated on nylon wool columns into nylon-adherent and nonadherent populations. Nylon-adherent cells were highly enriched for surface immunoglobulin (SIg) bearing B lymphocytes (95.5%) and nonadherent cells for SIg ne...

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Bibliographic Details
Published in:	American journal of veterinary research Vol. 38; no. 6; p. 873
Main Authors:	Paul, P S, Pomeroy, K A, Johnson, D W, Muscoplat, C C, Handwerger, B S, Soper, F F, Sorensen, D K
Format:	Journal Article
Language:	English
Published:	United States 01-06-1977
Subjects:	Animals B-Lymphocytes - microbiology Cattle Cattle Diseases - microbiology Cell Separation Female Lectins - pharmacology Leukemia Virus, Bovine - growth & development Lymphocyte Activation Lymphoma, Non-Hodgkin - microbiology Lymphoma, Non-Hodgkin - veterinary Retroviridae - growth & development Virus Replication
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Summary:	Bovine peripheral blood lymphocytes from a cow with persistent lymphocytosis were separated on nylon wool columns into nylon-adherent and nonadherent populations. Nylon-adherent cells were highly enriched for surface immunoglobulin (SIg) bearing B lymphocytes (95.5%) and nonadherent cells for SIg negative non-B cells, presumably T lymphocytes (96.3%). The B lymphocytes were found to be the major producers for bovine leukemia virus. A total of 39% of the B-enriched cells, surviving after 72 hours in culture, produced bovine leukemia virus as compared with 0.5% of the non-B cells.
ISSN:	0002-9645