Regulation of phosphate metabolism during intracellular lipid production in Rhodotorula gracilis

• Published in: Biotechnology letters Vol. 10; no. 10; pp. 745 - 750
• Main Authors: Sattur, A.P, Karanth, N.G, Divakar, S
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-01-1988
• Subjects: Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Fundamental and applied biological sciences. Psychology; glycerol 3-phosphate; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; lipids; metabolism; Microbiology; Mission oriented research; Mycology; N.M.R; phosphates; Physiology and metabolism; polyphosphate; production; regulation; Rhodotorula; Rhodotorula gracilis; Phosphates; Microorganism growth; Rhodotorula glutinis; Lipids; NMR spectrometry; Metabolism; Accumulation; Fungi; Regulation(control); Polyphosphoric acid; Production; Fungi Imperfecti; Intracellular; Thallophyta
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/BF01025293

super(31)P NMR spectra of intact cells of Rhodotorula gracilis showed pH dependent inorganic phosphate (P sub(i)) signals from two different compartments, namely, vacuole and cytoplasm. Clear distinction between growth phase and lipid accumulation phase was inferred by monitoring glycerol 3-phosphate and polyphosphate signals. The role of glycerol 3-phosphate in lipid production and the significance of polyphosphate accumulation during the same is discussed.