PCR versus ELISA in diagnosis of human toxoplasmosis in Jeddah, Saudi Arabia

In this study, polymerase chain reaction (PCR) was compared with detection of specific antibodies to Toxoplasma by ELISA. A total of 70 blood samples from patients with different clinical features were tested, 43/70 (61.4%) were serologically Toxoplasma-IgG positive meanwhile only 16/43 (37.2%) were...

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Bibliographic Details
Published in:	Journal of the Egyptian Society of Parasitology Vol. 38; no. 3; p. 707
Main Author:	Tonkal, Abdulkader M D
Format:	Journal Article
Language:	English
Published:	Egypt 01-12-2008
Subjects:	Animals Antibodies, Protozoan - blood DNA, Protozoan - analysis Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - standards Female Humans Immunocompromised Host Male Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Pregnancy Pregnancy Complications, Parasitic - diagnosis Reproducibility of Results Saudi Arabia Sensitivity and Specificity Toxoplasma - immunology Toxoplasma - isolation & purification Toxoplasmosis - diagnosis Saudi Arabia
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Summary:	In this study, polymerase chain reaction (PCR) was compared with detection of specific antibodies to Toxoplasma by ELISA. A total of 70 blood samples from patients with different clinical features were tested, 43/70 (61.4%) were serologically Toxoplasma-IgG positive meanwhile only 16/43 (37.2%) were approved to be positive by PCR. A total of 22 samples (31.6%) were positive by PCR, but only 16 of them were positive by ELISA. So, Tox-IgG indicated catching Toxoplasma infection but not enough with immune modulation to determine acute case, while PCR besides, being valuable in diagnosis showed Toxoplasma parasitemia.
ISSN:	1110-0583