Nucleotide sequence of bovine preprocathepsin B. A study of polymorphism in the protein coding region

Nucleotide sequence of bovine preprocathepsin B. A study of polymorphism in the protein coding region

A cDNA encoding bovine procathepsin B was isolated. The deduced amino acid sequence revealed that a stop (TAG) codon, instead of a Trp-257 codon (TGG), generates in bovine a cathepsin B precursor four amino acids shorter than in other species. Because micro-heterogeneities were previously reported i...

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Bibliographic Details
Published in:Biochimica et biophysica acta Vol. 1174; no. 3; p. 305
Main Authors: Mordier, S, Béchet, D, Roux, M P, Obled, A, Ferrara, M
Format: Journal Article
Language:English
Published: Netherlands 23-09-1993
Subjects:
Animals
Base Sequence
Cathepsin B - genetics
Cathepsins - genetics
Cattle
DNA - analysis
Enzyme Precursors - genetics
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Ribonucleases
Sequence Alignment
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Summary:A cDNA encoding bovine procathepsin B was isolated. The deduced amino acid sequence revealed that a stop (TAG) codon, instead of a Trp-257 codon (TGG), generates in bovine a cathepsin B precursor four amino acids shorter than in other species. Because micro-heterogeneities were previously reported in the cathepsin B primary structure, sequence polymorphism in the protein coding region was then investigated by PCR sequencing of genomic fragments and RNase protection assays. Experiments performed with 12-15 animals of three breeds did not reveal any difference with our cDNA sequence. We conclude that sequence polymorphism in bovine cathepsin B is a rare event, and can only result from the expression of different alleles of a unique gene.
ISSN:0006-3002
DOI:10.1016/0167-4781(93)90205-R