Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes: Results of an international slide-scoring exercise by the HUMN project

• Published in: Mutation research Vol. 534; no. 1; pp. 45 - 64
• Main Authors: Fenech, Michael, Bonassi, Stefano, Turner, Julie, Lando, Cecilia, Ceppi, Marcello, Chang, Wushou Peter, Holland, Nina, Kirsch-Volders, Micheline, Zeiger, Errol, Bigatti, Maria Paola, Bolognesi, Claudia, Cao, Jia, De Luca, Giuseppe, Di Giorgio, Marina, Ferguson, Lynnette R., Fucic, Aleksandra, Lima, Omar Garcia, Hadjidekova, Valeria V., Hrelia, Patrizia, Jaworska, Alicja, Joksic, Gordana, Krishnaja, A.P., Lee, Tung-Kwang, Martelli, Antonietta, McKay, Michael J., Migliore, Lucia, Mirkova, Ekaterina, Müller, Wolfgang-Ulrich, Odagiri, Youichi, Orsiere, Thierry, Scarfı̀, Maria Rosaria, Silva, Maria J., Sofuni, Toshio, Suralles, Jordi, Trenta, Giorgio, Vorobtsova, Irena, Vral, Anne, Zijno, Andrea
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10-01-2003; Elsevier
• Subjects: Analysis of Variance; Biological and medical sciences; Cell Nucleus Structures - genetics; Diverse techniques; DNA damage; Fundamental and applied biological sciences. Psychology; Human lymphocytes; Humans; HUMN project; International Cooperation; Laboratories; Lymphocytes - physiology; Lymphocytes - radiation effects; Male; Micronucleus; Micronucleus Tests - standards; Molecular and cellular biology; Nucleoplasmic bridges; Observer Variation; Poisson Distribution; Protocol; Reference Standards; Scoring; Variability; MULT, multinucleated; CBMN assay, cytokinesis-block micronucleus assay; MONO, mononucleated; DNA damage; Variability; Scoring; HUMN project; MN, micronucleus or micronuclei; BN, binucleated; Nucleoplasmic bridges; NDI, nuclear division index; Micronucleus; NPB, nucleoplasmic bridge; Human lymphocytes; Protocol; Human; Micronucleus test; Laboratory; Gamma irradiation; DNA; Lesion; Lymphocyte
• ISSN: 1383-5718; 0027-5107; 1879-3592
• DOI: 10.1016/S1383-5718(02)00248-6

One of the objectives of the HUman MicroNucleus (HUMN) project is to identify the methodological variables that have an important impact on micronucleus (MN) or micronucleated (MNed) cell frequencies measured in human lymphocytes using the cytokinesis-block micronucleus assay. In a previous study we had shown that the scoring criteria used were likely to be an important variable. To determine the extent of residual variation when laboratories scored cells from the same cultures using the same set of standard scoring criteria, an inter-laboratory slide-scoring exercise was performed among 34 laboratories from 21 countries with a total of 51 slide scorers involved. The results of this study show that even under these optimized conditions there is a great variation in the MN frequency or MNed cell frequency obtained by individual laboratories and scorers. All laboratories ranked correctly the MNed cell frequency in cells from cultures that were unirradiated, or exposed to 1 or 2
Gy of gamma rays. The study also estimated that the intra-scorer median coefficient of variation for duplicate MNed cell frequency scores is 29% for unexposed cultures and 14 and 11% for cells exposed to 1 and 2
Gy, respectively. These values can be used as a standard for quality or acceptability of data in future studies. Using a Poisson regression model it was estimated that radiation dose explained 67% of the variance, while staining method, cell sample, laboratory, and covariance explained 0.6, 0.3, 6.5, and 25.6% of the variance, respectively, leaving only 3.1% of the variance unexplained. As part of this exercise, nucleoplasmic bridges were also estimated by the laboratories; however, inexperience in the use of this biomarker of chromosome rearrangement was reflected in the much greater heterogeneity in the data and the unexplained variation estimated by the Poisson model. The results of these studies indicate clearly that even after standardizing culture and scoring conditions it will be necessary to calibrate scorers and laboratories if MN, MNed cell and nucleoplasmic bridge frequencies are to be reliably compared among laboratories and among populations.