Conjugated linoleic acid production by immobilized cells of Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus acidophilus

• Published in: Food chemistry Vol. 92; no. 1; pp. 23 - 28
• Main Authors: Lin, T.Y., Hung, T.-H., Cheng, T.-S.J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 2005; Elsevier
• Subjects: Biological and medical sciences; Chitosan; Conjugated linoleic acid; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; Immobilization; immobilized cells; Lactic acid bacteria; Lactobacillus acidophilus; Lactobacillus delbrueckii; Lactobacillus delbrueckii subsp. bulgaricus; optimization; Polyacrylamide; yields; Immobilization; Conjugated linoleic acid; Lactic acid bacteria; Chitosan; Polyacrylamide; Polyunsaturated fatty acid; Lipids; Immobilized cell; Lactobacillus delbrueckii; Linoleic acid; Lactobacillus delbrueckii subsp. bulgaricus; n-6 fatty acid; Production; Bacteria; Lactobacillaceae
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2004.07.003

Lactobacillus delbrueckii ssp.
bulgaricus (CCRC14009) and
L. acidophilus (CCRC14079), immobilized with chitosan and polyacrylamide, were tested for CLA production. A 10-ml aliquot of
L. delbrueckii ssp.
bulgaricus cell suspension (3.59
×
10
7 CFU/ml) was adsorbed to 0.5 g chitosan and polyacrylamide, mixed with 0.2 ml linoleic acid (0.9 g/ml), and incubated at 37 °C for 24 h at pH 5, 6, 7, and 8 for CLA production. CLA levels, produced by immobilized cells of
L.
delbrueckii ssp.
bulgaricus and
L. acidophilus with increasing cell counts to 1.08 and 1.28
×
10
10 CFU/ml, respectively, at optimal reaction pHs were evaluated. More CLA was formed at pH 8 of chitosan and pH 7 of polyacrylamide-immobilized
L.
delbrueckii ssp.
bulgaricus cell treatments. Increase in cell count resulted in higher CLA production. The adsorption of
L.
delbrueckii ssp.
bulgaricus cells onto polyacrylamide at pH 7 showed significant improvement in total CLA level. Results demonstrated a potential for enhancing CLA production through immobilization.