Short-Chain Fatty Acid Enemas Stimulate Plasminogen Activator Inhibitor-1 after Abdominal Aortic Graft Surgery: A Double-Blinded, Placebo-Controlled Study

• Published in: Thrombosis research Vol. 98; no. 5; pp. 361 - 366
• Main Authors: Mortensen, F.V, Jørgensen, B, Christiansen, H.M, Sloth-Nielsen, J, Wolff, B, Hessov, I
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Ltd 01-06-2000; Elsevier Science
• Subjects: Aged; Anti-Bacterial Agents - therapeutic use; Antigens - blood; Antigens - drug effects; Aorta, Abdominal - surgery; Aortic graft surgery; Arteriosclerosis - microbiology; Arteriosclerosis - surgery; Arteriosclerosis - therapy; Biological and medical sciences; Blood Vessel Prosthesis; Colitis, Ischemic - prevention & control; Colon - microbiology; Colon - pathology; Colonic ischaemia; Cross-linked fibrin degradation products; Double-Blind Method; Endotoxemia - drug therapy; Endotoxemia - etiology; Enema - methods; Fatty Acids, Volatile - pharmacology; Fatty Acids, Volatile - therapeutic use; Female; Fibrin Fibrinogen Degradation Products - drug effects; Fibrin Fibrinogen Degradation Products - metabolism; Fibrinolysis - drug effects; Humans; Male; Medical sciences; Middle Aged; Placebos; Plasminogen Activator Inhibitor 1 - metabolism; Plasminogen activator inhibitor-1; Short chain fatty acids; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Thromboembolism - prevention & control; Time Factors; Tissue Plasminogen Activator - blood; Tissue Plasminogen Activator - drug effects; Tissue Plasminogen Activator - immunology; Tissue-type plasminogen activator; Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels; Wound Infection - complications; XL-FDP, cross-linked fibrin degradation products; SCFAs, short-chain fatty acids; Cross-linked fibrin degradation products; Colonic ischaemia; Tissue-type plasminogen activator; IMA, inferior mesenteric artery; Plasminogen activator inhibitor-1; Aortic graft surgery; Short chain fatty acids; ELISA, enzyme-linked immunosorbent assay; t-PA, tissue-type plasminogen activator; PAI-1, plasminogen activator inhibitor-1; Human; Short chain; Homograft; Cardiovascular disease; Lipids; Fatty acids; Enema; Colonic disease; Prevention; Plasminogen activator inhibitor 1; Treatment; Ischemia; Surgery; Digestive diseases; Graft; Aorta; Complication; Colon
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/S0049-3848(00)00194-8

Colonic ischaemia may complicate aortic graft surgery with high mortality from associated colonic necrosis. Loss of the mucosal barrier function due to ischaemia may promote translocation of endotoxins with secondary systemic disseminated coagulation leading to multiple organ failure. Short-chain fatty acids (SCFAs) stimulate the microcirculation in the human rectum. The aim of this study was to investigate whether SCFA enemas influence systemic endotoxinaemia and fibrinolytic activity during and after elective aortic graft surgery for arteriosclerosis. Thirty-two patients were randomized to SCFA or placebo enemas twice daily from the day before surgery to 7 days after. Blood samples for endotoxin, plasminogen activator inhibitor-1 (PAI-1) activity, tissue-type plasminogen activator (t-PA) antigen, and cross-linked fibrin degradation products (XL-FDP) were drawn before, during, and 7 days after surgery. Four patients, two in each treatment group, developed postoperative endotoxinaemia. PAI-1 was significantly higher on days 2 and 4 in SCFA-treated patients, whereas t-PA was comparable between the groups. During the postoperative course, a progressive and near-identical XL-FDP increase was found in the two groups. In elective aortic graft surgery for arteriosclerosis, SCFA enemas likely stimulate systemic PAI-1 activity by promoting colonic tissue reperfusion following aortic unclamping. Endotoxinaemia and fibrinolytic shutdown are uncommon findings.