Genetic influences on reproduction of female red deer ( Cervus elaphus) : (2) Seasonal and genetic effects on the superovulatory response to exogenous FSH

• Published in: Animal reproduction science Vol. 59; no. 1; pp. 61 - 70
• Main Authors: Asher, G.W., O'Neill, K.T., Scott, I.C., Mockett, B.G., Pearse, A.J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 28-04-2000
• Subjects: Administration, Intravaginal; Animal Husbandry; Animals; Deer - genetics; Dynamics; Female; Follicle Stimulating Hormone - pharmacology; Genetics; Genotype; Progesterone - administration & dosage; Progesterone - pharmacology; Red deer; Seasonal; Seasons; Superovulation; Superovulation - drug effects; Superovulation - genetics; Red deer; Genetics; Seasonal; Dynamics; Superovulation
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/S0378-4320(00)00066-X

This study evaluated the influences of seasons and genotype on the superovulatory response to a standardised oFSH regimen in red deer (
Cervus elaphus scoticus) and its hybrids with either wapiti (
C.e. nelsoni) or Père David's (PD) deer (
Elaphurus davidianus). Adult red deer (
n=9), F
1 hybrid wapiti×red deer (
n=6), and maternal backcross hybrid PD×red deer (i.e.,
1
4
PD hybrid;
n=9) were kept together in the presence of a vasectomised stag for 13 months. At 6 weekly intervals, all hinds received a standardised treatment regimen used routinely to induce a superovulatory response in red deer hinds, with 10 consecutive treatments spanning an entire year. This involved synchronisation with intravaginal progesterone devices and delivery of multiple injections of oFSH (equivalent to 72 units NIH-FSH-S
1). Laparoscopy to assess ovarian response was performed 6–7 days after the removal of the devices. Both season and genotype had significant effects on ovulation rate (OR) and total follicular stimulation (TFS) (
P<0.05). For all the three genotypes, ovarian responses were highest from March to November (breeding season) and lowest in the period from December to January, inclusive. Mean OR for red deer hinds ranged from 3.7 to 1.8 during the breeding season, with no observable trend. All red deer hinds were anovulatory during December and January. A similar pattern occurred for
1
4
PD hybrids, although mean OR during the breeding seasons were twofold lower than for the red deer. For F
1 wapiti hybrids, the first two treatments in March and April resulted in the highest mean OR observed (15.6 and 11.7, respectively). Thereafter, mean values ranged between 6.3 and 4.7 for the remainder of the breeding season. Furthermore, mean OR of 3.0 and 0.5 were recorded in December and January, respectively. For the red deer and F
1 wapiti hybrids, between-hind variation in OR was not randomly distributed across the treatment dates, indicating that the individuals varied significantly in their ability to respond to oFSH, at least within a given season.
In conclusion, the study has shown that relative to red deer, F
1 wapiti hybrid hinds exhibit a higher sensitivity to oFSH, whereas
1
4
PD hybrid hinds have a lower sensitivity. However, individual variation within genotype was very marked. A seasonal effect was apparent for all genotypes, although some F
1 wapiti hybrid hinds exhibited ovulatory responses throughout the year.