Lactococcus lactis AbiD1 abortive infection efficiency is drastically increased by a phage protein

• Published in: FEMS microbiology letters Vol. 214; no. 2; pp. 283 - 287
• Main Authors: Bidnenko, Elena, Chopin, Marie-Christine, Ehrlich, S.Dusko, Anba, Jamila
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 10-09-2002
• Subjects: Abortive infection; Bacteriophage; Lactococcus lactis; Abortive infection; Bacteriophage; Lactococcus lactis
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(02)00898-4

Sensitivity of phage bIL66 to the AbiD1
Lactococcus lactis abortive infection mechanism was previously shown to be determined by the phage middle-time-expressed operon composed of four
orfs. Using spontaneous bIL66 mutants resistant to AbiD1, we established that this sensitivity is determined by the
orf1 encoded protein. Overproduction of Orf1 in trans in AbiD1
+ cells was shown to increase AbiD1 efficiency on both wild-type phage bIL66 and mutants resistant to AbiD1. Such an increase was not observed following overproduction of mutant Orf1. We propose that wild-type, but not a mutant Orf1, activates AbiD1 expression or activity.