Flow cytometric identification of Tfh13 cells in mouse and human

• Published in: Journal of allergy and clinical immunology Vol. 147; no. 2; pp. 470 - 483
• Main Authors: Chen, Jennifer S., Grassmann, Jessica D.S., Gowthaman, Uthaman, Olyha, Sam J., Simoneau, Tregony, Berin, M. Cecilia, Eisenbarth, Stephanie C., Williams, Adam
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01-02-2021
• Subjects: allergy; IgE; IL-13; IL-4; IL-5; intracellular cytokine staining; T follicular helper cells; Tfh13 cells; LN; RT; cIMDM; IgE; FACS; IMDM; RBC; FcR; BCL-6; Tfh; medLN; PMA; Tfh13 cells; IL-4; IL-5; allergy; FMO; IL-13; T follicular helper cells; TC; intracellular cytokine staining; NP-OVA; CPE; CXCR; PD-1
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2020.04.063

Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper Tfh13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying Tfh13 cells in both mice and humans.