Comparison of Mycoplasma agalactiae isolates by pulsed field gel electrophoresis, SDS-PAGE and immunoblotting

• Published in: FEMS microbiology letters Vol. 143; no. 2; pp. 259 - 265
• Main Authors: Tola, Sebastiana, Idini, Graziano, Manunta, Daniela, Casciano, Ida, Rocchigiani, angela M., Angioi, Antonio, Leori, Guido
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-10-1996
• Subjects: Epidemiological analysis; Immunoblotting; Mycoplasma agalactiae; Pulsed field gel electrophoresis; Mycoplasma agalactiae; Immunoblotting; Epidemiological analysis; Pulsed field gel electrophoresis
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/0378-1097(96)00323-0

We have analyzed 81 isolates of
Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in
M. agalactiae isolates was investigated analyzing hydrophobic membrane protein fractions by immunoblotting using pooled sheep antiserum from naturally infected sheep. Large restriction fragments obtained cleaving genomic DNAs with
SmaI,
NruI,
SalI,
XhoI,
BssHII and
KpnI were analyzed by pulsed field gel electrophoresis. Genetic analysis indicates that isolates are all similar without intraspecific differences. This homogeneity was confirmed by immunoblotting: 80 and 50 kDa antigens are present in all strains analyzed.