Glutathione, S-substituted glutathiones, and leukotriene C 4 as substrates for peptidylglycine α-amidating monooxygenase

• Published in: Archives of biochemistry and biophysics Vol. 412; no. 1; pp. 3 - 12
• Main Authors: Miller, Laura Aaron, Baumgart, Laura E, Chew, Geoffrey H, deLong, Mitchell A, Galloway, Lamar C, Jung, Kyung Woon, Merkler, Kathleen A, Nagle, Advait S, Poore, Derek D, Yoon, Cheol Hwan, Merkler, David J
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01-04-2003
• Subjects: Glutathione; Glutathione S-transferase; LTC 4; Peptidylglycine α-amidating monooxygenase; Xenobiotic detoxification; Xenobiotic detoxification; Peptidylglycine α-amidating monooxygenase; LTC 4; Glutathione S-transferase; Glutathione
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/S0003-9861(02)00730-0

The C-terminal α-amide moiety of most peptide hormones arises by the posttranslational cleavage of a glycine-extended precursor in a reaction catalyzed by bifunctional peptidylglycine α-amidating monooxygenase (PAM). Glutathione and the
S-alkylated glutathiones have a C-terminal glycine and are, thus, potential substrates for PAM. The addition of PAM to glutathione, a series of
S-alkylated glutathiones, and leukotriene C
4 results in the consumption of O
2 and the production of the corresponding amidated peptide and glyoxylate. This reaction proceeds in two steps with the intermediate formation of a C-terminal α-hydroxyglycine-extended peptide. Amidated glutathione (γGlu-Cys-amide) is a relatively poor substrate for glutathione
S-transferase with a
V/
K value that is 1.3% of that for glutathione. Peptide substrates containing a penultimate hydrophobic or sulfur-containing amino acid exhibit the highest (
V/
K)
app values for PAM-catalyzed amidation. The
S-alkylated glutathiones incorporate both features in the penultimate position with
S-decylglutathione having the highest (
V/
K)
app of the substrates described in this report.