Determination of donepezil hydrochloride in human and rat plasma, blood and brain microdialysates by HPLC with a short C 30 column

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 41; no. 1; pp. 201 - 206
• Main Authors: Nakashima, Kenichiro, Itoh, Keiko, Kono, Michio, Nakashima, Mihoko N., Wada, Mitsuhiro
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 11-04-2006
• Subjects: Donepezil hydrochloride; HPLC; Microdialysate; Plasma; Plasma; Donepezil hydrochloride; HPLC; Microdialysate
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2005.10.017

A simple and sensitive HPLC method with fluorescence (FL) detection for determination of donepezil (DP) in plasma and microdialysate samples was developed. A rapid isocratic separation of DP could be achieved by a short C
30 column using mobile phases of 25
mM citric acid/50
mM Na
2HPO
4 (pH 6.0)–CH
3CN (73:27%, v/v) containing 3.5
mM sodium 1-octanesulfonate for plasma and H
2O–CH
3CN–CH
3OH (80:17:3%, v/v/v) containing 0.01% acetic acid for microdialysate. The eluate was monitored at 390
nm with an excitation at 325
nm. The detection limits (S/N
=
3) of DP for human plasma, rat plasma and rat brain or blood microdialysates were 0.2, 1.0 and 2.1
ng/ml, respectively. Reproducible results could be obtained by using (±)-2-[(1-benzyl-piperidine-4-yl)ethyl]-5,6-dimethoxyindan-1-one hydrochloride as an internal standard (IS).
The method was successfully applied for monitoring of DP levels in rat plasma, blood and brain microdialysates and patient plasma.