400-MHz 1H-NMR spectroscopy of fucosylated tetrasialyl oligosaccharides isolated from normal and cirrhotic α 1-acid glycoprotein

• Published in: FEBS letters Vol. 238; no. 2; pp. 390 - 394
• Main Authors: Wieruszeski, J.M., Fournet, B., Konan, D., Biou, D., Durand, G.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 10-10-1988
• Subjects: 1H-NMR; Alcoholic cirrhosis; Ascitic Fluid - analysis; Asparagine-linked oligosaccharide; Carbohydrate Conformation; Carbohydrate Sequence; Carbohydrates - analysis; Fucose; Fucose - analysis; Humans; Liver Cirrhosis - metabolism; Magnetic Resonance Spectroscopy; Methylation; Molecular Sequence Data; Oligosaccharides - analysis; Orosomucoid - analysis; Sialic Acids - analysis; α 1-Acid glycoprotein; n, normal; 1H-NMR; c, cirrhotic; Alcoholic cirrhosis; Asparagine-linked oligosaccharide; OMe, O-methyl; α 1-AGP, α 1-acid glycoprotein; GlcNAcNMeol, N-acetyl- N- methylglucosaminitol; α 1-Acid glycoprotein; Fucose
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(88)80518-0

The comparative study of fucosylated tetrasialyl-oligosaccharides isolated from normal and cirrhotic α
1-AGP was performed using permethylation and 400-MHz
1H-NMR spectroscopy. These results clearly show the tetraantennary structure of these two oligosaccharides with hyperfucosylation for the tetrasialylated fraction from cirrhotic α
1-AGP. In the latter oligosaccharide the simultaneous presence on two antennae (7 and 7′) of the sialosyl Lewis X determinant NeuAc-(α2–3) Gal(β1–4) [Fuc(αl–3)l GlcNAc has been observed. Moreover the 5 and 5′ antennae were α2–6 sialylated but without fucose.