Transcription Factor ETS1 Promotes Glioma Cell Growth by Activating LncRNA XIST

• Published in: Zhongliu fangzhi yanjiu Vol. 51; no. 5; pp. 328 - 335
• Main Authors: Luo, Ran, Luo, Wenyi, Lu, Mingkai, Zhou, Meng, Liu, Yanting, Tian, Chunlei
• Format: Journal Article
• Language: Chinese
• Published: Tianjin China Anti-Cancer Association 01-05-2024; Magazine House of Cancer Research on Prevention and Treatment
• Subjects: Apoptosis; BAX protein; Bcl-2 protein; Binding sites; Bioinformatics; Cell growth; Cell proliferation; Cholecystokinin; Chromatin; Ets-1 protein; ets1; Gene expression; Glioma; Glioma cells; Immunohistochemistry; Immunoprecipitation; lncrna xist; Non-coding RNA; Polymerase chain reaction; Proteins; Reporter gene
• ISSN: 1000-8578
• DOI: 10.3971/j.issn.1000-8578.2024.23.1055

Objective To explore the biological function and downstream mechanism of ETS1 in glioma.Methods Bioinformatics and immunohistochemistry were used to analyze the differential expression characteristics of ETS1 in gliomas; qRT-PCR was employed to detect the expression level of ETS1 mRNA and lncRNA X-inactive specific transcript（XIST）. CCK-8 and 5-ethyl-2 ′-deoxyuridine experiments were conducted to detect cell growth. Western blot was used to detect the expression of apoptosis-related proteins（Bax, Bak, Bcl-2）. PROMO database was utilized to predict the binding sites between ETS1 and XIST promoter. Dual-luciferase reporter gene assay and chromatin immunoprecipitation-quantitative polymerase chain reaction assays were performed to verify the binding relationship between ETS1 and the XIST promoter region. cBioPortal database was used to analyze the correlation between the expression of ETS1 mRNA and XIST in glioma tissues. Results The expression levels of ETS1 mRNA and protein were significantly upregulated in gl