Outer membrane protein C (ompC) gene as target for diagnosis of Salmonella spp. using polymerase chain (PCR) reaction

• Published in: Al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 15; no. 1; pp. 13 - 17
• Main Authors: Jawad, Ala Abd al-Kazim, al-Janabi, Hashim Rahim Tarish, al-Jarakh, Ala Hani
• Format: Journal Article
• Language: Arabic; English
• Published: a-Qadisiyah, Iraq al-Qadisiyah University, College of Veterinary Medicine 01-06-2016; College of Veterinary Medicine
• ISSN: 1818-5746; 2313-4429

A total of 1200 different specimens collected from human and animals sources. 600 specimens from patients suffering from diarrhea who were admitted to Al-Diwaniya Teaching Hospital for Maternity and Children. 600 specimens from gall bladder (bile) of cattle from Al-Diwaniya slaughter house. This study was conducted during the period that extended from May 2013 to April 2014. Salmonella spp. were isolated and identified using bacterial culturing on selective media, biochemical, API 20E, serotyping by polyvalent and conformation by PCR. Polymerase chain reaction (PCR) was used to detect ompC gene encoding for biosynthesis of outer membrane protein C of Salmonella genus. The results revealed that the rate of Salmonella isolates was 0.5% (3/600) from human and 1% (6/600) from animals. The PCR technique revealed that 9 isolates of Salmonella spp. were contain ompC gene (DNA amplification showed one distinct band with molecular weight of 204 bp when electrophorised on agarose gel).The results of this study revealed that the PCR technique had a high specificity in detection of Salmonella spp., in comparison to culture and biochemical test, Mini API 20 E and serological tests. The present study found no significant differences between human and animal isolates.