The Anti-Melanogenic Effects of Ganodermanontriol from the Medicinal Mushroom Ganoderma lucidum through the Regulation of the CREB and MAPK Signaling Pathways in B16F10 Cells

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 16; p. 3976
• Main Authors: Park, Che-Hwon, Oh, Youn-Lee, Shin, Ju-Hyeon, Park, Young-Jin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-08-2024; MDPI
• Subjects: Animals; B16F10 melanoma cells; Biosynthesis; Cell Line, Tumor; Chromatography; Cyclic AMP Response Element-Binding Protein - metabolism; Enzymes; Free radicals (Chemistry); Ganoderma lucidum; ganodermanontriol; Kinases; MAP Kinase Signaling System - drug effects; Melanins - biosynthesis; Melanins - metabolism; Melanoma; Melanoma, Experimental - metabolism; Melanoma, Experimental - pathology; Mice; microphthalmia-associated transcription factor; Microphthalmia-Associated Transcription Factor - metabolism; Monophenol Monooxygenase - antagonists & inhibitors; Monophenol Monooxygenase - metabolism; Phosphorylation; Phosphorylation - drug effects; Protein expression; Proteins; Radiation; Reishi - chemistry; Signal Transduction - drug effects; Skin; Transcription factors; tyrosinase; tyrosinase-related proteins; Tyrosine; South Korea; microphthalmia-associated transcription factor; B16F10 melanoma cells; Ganoderma lucidum; tyrosinase-related proteins; ganodermanontriol; tyrosinase
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29163976

, a member of the Basidiomycetes family, is attracting attention for its medicinal potential due to its biological activity and the presence of numerous bioactive compounds. Although it is known that extracts of this mushroom inhibit melanin production, there are few reports on a single substance associated with this effect. In this study, we identified ganodermanontriol (GT), a novel compound from
, that effectively inhibited melanin biosynthesis in B16F10 cells. GT inhibits melanin production by suppressing the expression of cellular tyrosinase proteins and microphthalmia-related transcription factor (MITF). Furthermore, GT affects the phosphorylation of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) and mitogen-activated protein kinase (MAPK) signaling molecules, which are involved in melanogenesis in B16F10 cells. Finally, the biosynthesis of GT and other substances by
was evaluated using HPLC analysis. Thus, this study revealed the mechanism by which GT in
inhibits melanin production in B16F10 cells, and these findings will contribute to promoting the potential use of this mushroom in the future.