Significance of Duodenal Prolactin Receptor Modulation by Calcium and Vitamin D in Sulpiride-Induced Hyperprolactinemia

• Published in: Medicina (Kaunas, Lithuania) Vol. 60; no. 6; p. 942
• Main Authors: Radojkovic, Danijela Branislav, Pesic, Milica, Radojkovic, Milan, Vukelic Nikolic, Marija, Jevtovic Stoimenov, Tatjana, Radenkovic, Sasa, Ciric, Vojislav, Basic, Dijana, Radjenovic Petkovic, Tatjana
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-06-2024; MDPI
• Subjects: Alfacalcidol; Analysis; Animals; Antipsychotics; Bones; Calcifediol; calcium; Calcium - metabolism; Cold; Drug dosages; Duodenum - drug effects; Duodenum - metabolism; Enzymes; Euthanasia; Female; Gastrointestinal system; Gene expression; Gene Expression - drug effects; Genes; Health aspects; Homeostasis; Hyperprolactinemia; Hyperprolactinemia - chemically induced; Hyperprolactinemia - drug therapy; Life sciences; medicamentous hyperprolactinemia; Osteoporosis; Prolactin; prolactin receptors; Psychotropic drugs; Rats; Rats, Wistar; Receptors, Prolactin - metabolism; Small intestine; Sulpiride - pharmacology; Urine; Vertebrae; Vitamin D; Vitamin D - pharmacology; Vitamin D - therapeutic use; Serbia; United States > US; calcium; prolactin receptors; medicamentous hyperprolactinemia; vitamin D
• ISSN: 1648-9144; 1010-660X; 1648-9144
• DOI: 10.3390/medicina60060942

Hyperprolactinemia, as a potential side-effect of some antipsychotic medications, is associated with decreased bone density and an increased risk of fractures. This study investigates whether calcium and vitamin D supplementation affects prolactin receptor (
gene expression in the duodenum, vertebrae, and kidneys of female rats with sulpiride-induced hyperprolactinemia.
Twenty-one-week-old female Wistar rats were assigned to three groups: Group S consisted of ten rats who received sulpiride injections (10 mg/kg) twice daily for 6 weeks; Group D (10 rats) received daily supplementation of 50 mg calcium and 500 IU vitamin D along with sulpiride for the last 3 weeks; and Group C consisting of seven age-matched nulliparous rats serving as a control group. Real-time PCR was used to assess
gene expression in the duodenum, vertebrae, and kidneys.
In Group S, Prlr gene expression was notably decreased in the duodenum (
< 0.01) but elevated in the vertebrae and kidneys compared to Group C. Conversely, Group D exhibited significantly increased Prlr expression in the duodenum (
< 0.01) alongside elevated expression in the vertebrae and kidneys.
In sulpiride-induced hyperprolactinemia, decreased Prlr gene expression in the duodenum may lead to reduced intestinal calcium absorption. Consequently, prolactin may draw calcium from the skeletal system to maintain calcium balance, facilitated by increased Prlr gene expression in the vertebrae. However, vitamin D supplementation in sulpiride-induced hyperprolactinemia notably enhances Prlr gene expression in the duodenum, potentially ameliorating intestinal calcium absorption and mitigating adverse effects on bone health.