Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba dispar

• Published in: Iranian journal of parasitology Vol. 7; no. 1; pp. 97 - 103
• Main Authors: Nazemalhosseini-Mojarad, E, Azimirad, M, Nochi, Z, Romani, S, Tajbakhsh, M, Rostami-Nejad, M, Haghighi, A, Zali, Mr
• Format: Journal Article
• Language: English
• Published: Iran Tehran University of Medical Sciences 01-01-2012
• Subjects: Bacteria; Diversity; Entamoeba Dispar; Genes; Genetic diversity; Iran; Locus A-L; Microbiology; Original; Parasites; STRs; Studies; Locus A-L; Iran; STRs; Entamoeba dispar; Diversity
• ISSN: 1735-7020; 2008-238X

A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates.
A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced. The sequences obtained were edited manually and aligned using Gene Runner software.
With sequencing of PCR products a reliable genetic diversity in size, number and position of the repeat units were observed among the Iranian E. dispar isolates in locus A-L gene. Sequences showed variation in length from 448bp to 507bp and seven distinct types were identified.
The genetic diversity of loci like A-L shows them to be suitable for epidemiological studies such as the characterization of the routes of transmission of these parasites in Iran.