Photocatalytic, antimicrobial and antibiofilm activities of MgFe2O4 magnetic nanoparticles

• Published in: Scientific reports Vol. 14; no. 1; p. 12877
• Main Authors: El-Khawaga, Ahmed M., Ayman, Mohamed, Hafez, Omar, Shalaby, Rasha E.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05-06-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/61/168; 639/925/350; 639/925/350/354; 704/172; Ambient temperature; Antibacterial activity; Antibiofilm activity; Antimicrobial activity; Azo dyes; Bacteria; Biodegradation; Biofilms; Carbol fuchsin; Color removal; Crystals; Decolorization; Dilution tests; E coli; Fourier transforms; Gram-negative bacteria; Gram-positive bacteria; Humanities and Social Sciences; Infrared spectroscopy; Irradiation; Magnesium; Magnetic nanoparticles; MgFe2O4 nanoparticles; Microscopy; Morphology; multidisciplinary; Nanocomposites; Nanoparticles; Optical properties; Photocatalysis; Photodegradation; Scanning electron microscopy; Science; Science (multidisciplinary); Spectroscopy; Spectrum analysis; Transmission electron microscopy; Ultraviolet radiation; X-ray diffraction; Antimicrobial activity; nanoparticles; Carbol fuchsin; Photocatalysis; Magnetic nanoparticles; Antibiofilm activity; MgFe; O
• ISSN: 2045-2322
• DOI: 10.1038/s41598-024-62868-5

This study reports the antibacterial and antibiofilm activities of Magnesium ferrite nanoparticles (MgFe
2
O
4
) against gram-positive and gram-negative bacteria. The photocatalytic degradation of Carbol Fuchsin (CF) dye (a class of dyestuffs that are resistant to biodegradation) under the influence of UV-light irradiation is also studied. The crystalline magnesium ferrite (MgFe
2
O
4
) nanoparticles were synthesized using the co-precipitation method. The morphology of the resulting nanocomposite was examined using scanning electron microscopy (SEM), while transmission electron microscopy (TEM) was employed for further characterization of particle morphology and size. Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) were utilized to analyze the crystalline structure, chemical composition, and surface area, respectively. Optical properties were evaluated using UV–Vis spectroscopy. The UV-assisted photocatalytic performance of MgFe
2
O
4
nanoparticles was assessed by studying the decolorization of Carbol fuchsin (CF) azo dye. The crystallite size of the MgFe
2
O
4
nanoparticles at the (311) plane, the most prominent peak, was determined to be 28.5 nm. The photocatalytic degradation of 10 ppm CF using 15 mg of MgFe
2
O
4
nanoparticles resulted in a significant 96% reduction after 135 min at ambient temperature (25 °C) and a pH value of 9. Additionally, MgFe
2
O
4
nanoparticles exhibited potent antibacterial activity against
E. coli
and
S. aureus
in a dose dependent manner with maximum utilized concentration of 30 µg/ml. Specifically, MgFe
2
O
4
nanoparticles demonstrated substantial antibacterial activity via disk diffusion and microbroth dilution tests with zones of inhibition and minimum inhibitory concentrations (MIC) for
E. coli
(26.0 mm, 1.25 µg/ml)
and S. aureus
(23.0 mm, 2.5 µg/ml), respectively. Moreover, 10.0 µg/ml of MgFe
2
O
4
nanoparticles elicited marked percent reduction in biofilm formation by
E. coli
(89%) followed by
S. aureus
(78.5%) after treatment. In conclusion, MgFe
2
O
4
nanoparticles demonstrated efficient dye removal capabilities along with significant antimicrobial and antibiofilm activity against gram-positive and gram-negative bacterial strains suggesting their potential as promising antimicrobial and detoxifying agents.