Detection of Mycoplasma gallisepticum in broiler chickens by PCR

• Published in: Open veterinary journal (Tripoli, Libya) Vol. 12; no. 3; pp. 329 - 334
• Main Authors: Mahmmoud, Ebtehal N, Hamad, Mohammad A, Khudhur, Zahraa N
• Format: Journal Article
• Language: English
• Published: Tripoli, Libya Faculty of Veterinary Medicine 01-01-2022; Tripoli University
• Subjects: broiler; chronic respiratory disease; mycoplasma gallisepticum; Original Research; pcr
• ISSN: 2226-4485; 2218-6050
• DOI: 10.5455/OVJ.2022.v12.i3.4

BackgroundMycoplasma is a significant microorganism of poultry, which can cause respiratory infections and synovial inflammation, bringing about huge financial misfortunes to poultry workmanship worldwide. AimThe goal of existing research was to determine the infection rate of Mycoplasma gallisepticum (MG) from chronic respiratory disease cases among broilers fields in Mosul/ Iraq using the polymerase chain reaction (PCR) technique. MethodsAll 92 lungs samples were collected from broilers with classical respiratory signs in different regions of the Nineveh governorate for 3 months from February to April 2021. ResultsPCR tests were performed using two couple primers, one for the qualitative amplification of 16S rRNA genes (285 base pairs) in Mycoplasma spp. and the second couple for the detection of M. gallisepticum (580 base pairs). Among the samples obtained from broilers, 87 (94.7%) were positive for Mycoplasma and 79 (85.9%) were positive for M. gallisepticum. ConclusionOur results showed that MG infection in broiler chickens leads to serious clinical symptoms and severe lesions. The rate of Mycoplasma isolation in this study is high despite the short lifespan of broiler chickens.