Design and Construction of a Cloning Vector Containing the hspX Gene of Mycobacterium tuberculosis

• Published in: Reports of biochemistry and molecular biology Vol. 5; no. 1; pp. 46 - 50
• Main Authors: Yaghoubi, Atieh, Aryan, Ehsan, Zare, Hosna, Alami, Shadi, Teimourpour, Roghayeh, Meshkat, Zahra
• Format: Journal Article
• Language: English
• Published: Iran Varastegan Institute for Medical Sciences 01-10-2016
• Subjects: Cloning; DNA vaccine; hspX; Mycobacterium tuberculosis; Short; DNA vaccine; hspX; Mycobacterium tuberculosis; Cloning
• ISSN: 2322-3480; 2322-3480

Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing the
.
First, an
fragment was amplified by PCR and cloned into plasmid pcDNA3.1(+) and recombinant vector was confirmed.
A 435 bp
fragment was isolated. The fragment was 100% homologous with
of M. tuberculosis strain H37Rv in GenBank.
In this study, the cloning vector pcDNA3.1(+), containing a 435-bp
fragment of
, was constructed. This could be used as a DNA vaccine to induce immune responses in animal models in future studies.