The Efficacy of Potential Probiont Bacillus amyloliquefaciens Strain L11 in Protecting Artemia Nauplii and Blue Crab Juveniles against Vibrio harveyi Infection

• Published in: Journal of pure & applied microbiology : an international research journal of microbiology Vol. 13; no. 2; pp. 923 - 931
• Main Authors: Azrin, N.A.R, Yuzine, E, Ina-Salwany, M.Y, Karim, Murni
• Format: Journal Article
• Language: English
• Published: Oriental Scientific Publishing Company 01-06-2019; Journal of Pure and Applied Microbiology
• Subjects: artemia nauplii; bacillus amyloliquefaciens; blue crab juveniles; vibrio harveyi; Malaysia
• ISSN: 0973-7510; 2581-690X
• DOI: 10.22207/JPAM.13.2.29

In this study, Bacillus amyloliquefaciens strain L11 was used as potential probiont in preliminary in vivo assay using Artemia and blue crab juveniles as final host. Strain L11 at [10.sup.8] CFU [mL.sup.-1] was found able to penetrate into gnotobiotic Artemia as early as 6h exposure. The survival rate of Artemia incubated with strain L11 at concentration of [10.sup.6] CFU [ml.sup.-1] and challenged with V. harveyi was 62 [+ or -] 1%. The numbers of Vibrios in Artemia were reduced at the end of the challenge assay. The in vivo assay using blue crab juveniles, demonstrated that strain L11 at [10.sup.6] CFU [ml.sup.-1] showed significant survival (42 [+ or -] 1 %) compared with group challenged with V. harveyi with no probiont added (12 [+ or -] 1 %) after five days of exposure. Strain L11 also able to reduce the number of Vibrios and increased the weight of the juveniles. Keywords: Bacillus amyloliquefaciens, Artemia nauplii, blue crab juveniles, Vibrio harveyi.