Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome

• Published in: Ukrainian biochemical journal Vol. 86; no. 3; pp. 98 - 106
• Main Authors: Hubs'kyĭ, Iu I, Bukhtiarova, T A, Horiushko, H H, Litvinova, N V, Paramonova, H I, Kurapova, T M, Velychko, O M, Babenko, L P
• Format: Journal Article
• Language: Ukrainian
• Published: Ukraine National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 01-05-2014
• Subjects: Acetaminophen - metabolism; Acetaminophen - pharmacology; acute nociceptive pain; Acute Pain - prevention & control; Analgesics, Non-Narcotic - metabolism; Analgesics, Non-Narcotic - pharmacology; Animals; Antipyrine - metabolism; Antipyrine - pharmacology; Aspirin - metabolism; Aspirin - pharmacology; Calorimetry; Cell Fractionation; Erythrocyte Membrane - chemistry; Erythrocyte Membrane - metabolism; erythrocytes membranes; Fluorescent Dyes; fluorescent probing; Indomethacin - metabolism; Indomethacin - pharmacology; Ketoprofen - metabolism; Ketoprofen - pharmacology; Ketorolac - metabolism; Ketorolac - pharmacology; Lipid Bilayers - chemistry; Lipid Bilayers - metabolism; Lipid Peroxidation - drug effects; Male; microcalorimetry; non-opioid analgesics; Orphenadrine - metabolism; Orphenadrine - pharmacology; oxidative stress; Pain Measurement; Rats; Spectrometry, Fluorescence; Sulfonamides - metabolism; Sulfonamides - pharmacology
• ISSN: 2409-4943; 2413-5003
• DOI: 10.15407/ubj86.03.098

Methods of fluorescent probing, spectrophotometry and microcalorimetry were applied to investigate the alterations in biophysical parameters of erythrocytes membranes, and specifically microviscosity, surface charge, molecular organization of lipid bilayer and lipid-protein interactions under conditions of acute pain syndrome produced by experimental chemical lesion. The distinctive features of non-opiod analgesics interactions and binding to the erythrocytes membranes of rats subjected to acute nociceptive pain accompanied with oxidative stress development were investigated. The abilities of analgesics under research, and namely paracetamol, aspirin, phenazone, ketorolac, pyrodazole, ketoprofenum, natrium mefenaminate, indometacin, nimesulide to make up physico-chemical complexes with lipoperoxidation modified erythrocytes surface and protein-lipid bilayer showed marked changes. The significance of oxidative damage of biophase under conditions of acute pain syndrome for analgesics effective pharmacodynamics and pharmacokinetics realization is under consideration.