Antidiabetic potential of mucilage fraction extracted from Astragalus gyzensis seeds

• Published in: European Journal of Biological Research Vol. 13; no. 1; pp. 18 - 30
• Main Authors: Tedjani, Aicha, Boual, Zakaria, Hadj, Mohamed Didi Ould El, Lanez, Touhami, Belkhalfa, Hakim, Alaoui-Talibi, Zainab El, Modafar, Cherkaoui El, Abdelkafi, Slim, Fendri, Imen, Cerf, Didier Le, Dubessay, Pascal, Delattre, Cédric, Guillaume, Pierre, Michaud, Philippe
• Format: Journal Article
• Language: English
• Published: Poznan European Journal of Biological Research 01-01-2023; TMKarpiński Publisher; TMKarpinski Publisher, Tomasz M. Karpiński
• Subjects: Antidiabetic; Astragalus gyzensis; Astragalus gyzensis Bunge; Biological activity; Biotechnology; Carbohydrates; Chromatography; Colorimetry; Diabetes mellitus; Dietary supplement fiber; Dietary supplements; Galactomannans; Galactose; Industrial applications; Infrared spectroscopy; Life Sciences; Mannose; Mucilage; Phenolic compounds; Polysaccharides; Seeds; Thin-layer chromatography; Uronic acid; α-Amylase; α-Glucosidase; Astragalus gyzensis Bunge; Mucilage; Dietary supplement fiber; Antidiabetic; Galactomannans
• ISSN: 2449-8955; 2449-8955
• DOI: 10.5281/zenodo.7618530

The objective of the current work is to extract a new mucilage fraction from Astragalus gyzensis Bunge. seeds, which are collected from the El-Oued province (septentrional Algerian Sahara) and evaluated for their antidiabetic potential. The mucilage fraction is obtained using hot water extraction followed by alcoholic precipitation of polysaccharides by cold ethanol (96%). The primary investigation was performed by describing the main structural features of the extract through colorimetric assays, Fourier-transform infrared spectroscopy and thin-layer chromatography analysis using two systems. Biological activity was also monitored by antidiabetic activity by testing the inhibition of a-amylase and a-glucosidase enzymes in vitro. The extraction yield was 20.69%. The chemical composition mainly consisted of 78.60±0.29% carbohydrates, among them 63.92±0.67% neutral sugar, 15.78±0.76% uronic acid, 8.08±0.04% proteins and 2.57±0.05% phenolic compounds. The results obtained by thin-layer chromatography analysis showed the dominance of mannose and galactose. Fourier-transform infrared spectrum showed characteristic bands expected galactomannans. The investigations highlighted the antihyperglycemic effect in a dose-dependent manner by the inhibition of the aamylase enzyme (IC50=0.8±0.005 mg/mL). These factors make it suitable for the industrial application of dietary supplement fiber made for diabetic individuals.