THE PANCREAS OF NEWBORN RABBITS AS A SOURCE OF PROGENITOR CELLS

THE PANCREAS OF NEWBORN RABBITS AS A SOURCE OF PROGENITOR CELLS

The newborn rabbit pancreas was used as a source of ductal epithelial cells. Our approach includes the cultiva- tion of pancreatic tissue microfragments without preliminary islet and duct isolation. Under ordinary conditions we found the epithelial pancreatic cell monolayer. The cultivated monolayer...

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Bibliographic Details
Published in:Vestnik transplantologii i iskusstvennykh organov Vol. 13; no. 1; pp. 61 - 64
Main Authors: L. A. Kirsanova, N. V. Baranova, N. N. Skaletsky, V. A. Zaidenov, G. N. Bubentsova, I. A. Pushkova
Format: Journal Article
Language:Russian
Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 01-06-2011
Subjects:
ductal cell monolayer
progenitor cells
rabbit pancreas
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Summary:The newborn rabbit pancreas was used as a source of ductal epithelial cells. Our approach includes the cultiva- tion of pancreatic tissue microfragments without preliminary islet and duct isolation. Under ordinary conditions we found the epithelial pancreatic cell monolayer. The cultivated monolayer cells were shown by immunofluo- rescence to express special ductal cytokeratin 19 and were insulin- and glucagon-negative for 7–15 days.
ISSN:1995-1191
DOI:10.15825/1995-1191-2011-1-61-64