PROTEOMIC MASS-SPECTROMETRIC ANALYSIS OF FRESHLY ISOLATED ESCHERICHIA COLI STRAINS

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii Vol. 92; no. 3; pp. 83 - 88
• Main Authors: Gapon, M N, Telesmanich, N R, Ternovskaya, L N, Chaika, S O, Chaika, I A, Mikashinovich, Z I, Chernukhina, T B, Tverdokhlebova, T I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) Central Research Institute for Epidemiology 01-05-2015
• Subjects: biotyping; escherichia coli; Escherichia coli - genetics; Escherichia coli - pathogenicity; Escherichia coli Proteins - biosynthesis; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Humans; Intestines - microbiology; Mass Spectrometry; mass-peak-list; Proteomics
• ISSN: 0372-9311; 2686-7613

Time-of-flight mass-spectrometric analysis of colon bacilli populations freshly isolated from humans for identification of proteome markers, that are characteristic for hemolytic and non-hemolytic strains, E.coli--associants of opportunistic bacteria.
20 strains of monopopulation of hemolytic E.coli; 100 strains of monopopulation of non-hemolytic E. coli; 15 non-hemolytic strain, isolated from association with opportunistic bacteria. Protein profiling on MALDI-TOF MS Autoflex "Bruker Daltonik" mass-spectrometer.
Value within species for mass-charge peak with 100% intensity of 9000 Da could be a differential sign for isolation of hemolytic E.coli during mass-spectrometric biotyping.
Proteomic characteristics of strains from various populations within E.coli are given. Taxon-specific markers for proteomic differentiation of E.coli populations are established.