Clinical, laboratory and morphological comparisons in cellular alteration under conditions of hypoxia

• Published in: Arkhiv patologii Vol. 86; no. 5; p. 42
• Main Authors: Semenova, N Yu, Zinserling, V A, Artyukhina, Z E, Marichev, A O, Radovsky, A M, Bautin, A E
• Format: Journal Article
• Language: English; Russian
• Published: Russia (Federation) 2024
• Subjects: Animals; Apoptosis; Basic Helix-Loop-Helix Transcription Factors - metabolism; Caspase 3 - metabolism; Hypoxia - metabolism; Hypoxia - pathology; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Kidney - metabolism; Kidney - pathology; Liver - metabolism; Liver - pathology; Swine; alterative changes; hypoxia; kidneys; dystrophic changes; liver
• ISSN: 0004-1955
• DOI: 10.17116/patol20248605142

To compare structural changes in tissues with clinical and laboratory parameters and immunohistochemical determination of proteins HIF1α, HIF2α, caspase-3 during cellular alteration under conditions of hypoxia in the liver and kidneys in an experiment on pigs.
Laboratory parameters related to the state of gas exchange (decrease in partial pressure of arterial blood oxygen (PaO2), arterial blood saturation, lactate level, kidney function (creatinine, urea) and liver (ALT, AST, bilirubin) in 18 animals were analyzed in comparison with the results of a morphological study. Histological examination evaluated alterative and inflammatory tissue changes of varying severity, and also determined the expression of transcription factors HIF1α and HIF2α and a marker of apoptosis - caspase-3. The ratio between laboratory parameters and structural changes was assessed individually for each animal.
In the liver, a statistically significant dependence of the content of HIF2α protein in cells on the severity of dystrophic changes and serum lactate levels was revealed. A statistically significant correlation was shown between an increase in transaminase and bilirubin levels and the severity of alterative changes in liver tissue. However, there was no significant relationship between the number of caspase-3 positive cells and the severity of dystrophic changes. A statistically significant correlation was found between creatinine and urea levels and the severity of alterative changes in kidney tissues. With significant dystrophic changes, a statistically significant dependence of the expression of HIF2α and caspase-3 proteins and a very high correlation of caspase-3 - lactate indicators were revealed.
A significant correlation was shown between histological changes in tissues and clinical and laboratory parameters. Severe hypoxia with lactate accumulation directly affects the integrity and function of cells, which manifests itself in structural changes. Based on the results of a comparative study, it can be concluded that the assessment of alterative changes in liver and kidney tissues is important.