Description of Millerago gen. nov. based on taxogenomic analysis, with two new species, Millerago phaffii f.a., sp. nov. and Millerago galiae f.a., sp. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 74; no. 10
• Main Authors: García-Acero, Angela M, Batista, Thiago M, Souza, Gisele F L, Santos, Ana Raquel O, Souza, Daniela L, Franco, Glória R, Velásquez-Lozano, Mario E, Yamamoto, Daichi, Toki, Wataru, Lachance, Marc-André, Rosa, Carlos A
• Format: Journal Article
• Language: English
• Published: England 01-10-2024
• Subjects: Animals; Candida - classification; Candida - genetics; Candida - isolation & purification; Colombia; DNA, Fungal - genetics; DNA, Ribosomal Spacer - genetics; Insecta - microbiology; Japan; Mycological Typing Techniques; Phylogeny; Plant Bark - microbiology; Quercus - microbiology; Saccharomycetales - classification; Saccharomycetales - genetics; Saccharomycetales - isolation & purification; Sequence Analysis, DNA; Colombia; Japan; Millerago phaffii sp. nov; new yeast genus; Quercus spp; Millerago galiae sp. nov
• ISSN: 1466-5034
• DOI: 10.1099/ijsem.0.006565

Four yeast isolates obtained from tree bark and fermenting sap of
spp. and insects in Colombia and Japan were phylogenetically related to
based on analyses of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene. The novel species differs from
by 20 nt substitutions and 5 indels in the D1/D2 sequences. A phylogenomic analysis suggested that these species are related to
, the genus
and a small clade containing
,
and
. Our genomic analyses suggest that the novel species and
should be separated in a novel yeast genus. We propose the genus
gen. nov. to accommodate these species and the species
f.a., sp. nov. (CBS 18021
; MycoBank MB856172) to accommodate the Colombian and Japanese isolates. The Colombian isolate of
differs from the Japanese isolates by three nt substitutions and one indel and two substitutions and one indel in the ITS and D1/D2 sequences, respectively, showing that they were conspecific. We also propose the new species
sp. nov. to validate this species according to the rules of the International Code of Nomenclature for algae, fungi and plants.