Uncommon non-oncogenic HPV genotypes, TP53 and MDM2 genes polymorphisms in HIV-infected women in Southern Brazil

• Published in: The Brazilian journal of infectious diseases Vol. 18; no. 6; pp. 643 - 650
• Main Authors: Entiauspe, Ludmila Gonçalves, Seixas, Fabiana Kömmling, Nunes, Emily Montosa, Rodrigues, Fernanda Martins, Dellagostin, Odir A., Collares, Tiago, da Silveira, Mariângela Freitas
• Format: Journal Article
• Language: English
• Published: Brazil Elsevier Editora Ltda 01-11-2014; Contexto; Elsevier; Brazilian Society of Infectious Diseases
• Subjects: Adolescent; Adult; Brazil; Cervical cancer; Cross-Sectional Studies; Development and progression; DNA, Viral - genetics; Epidemiology; Female; Genetic aspects; Genetic polymorphisms; Genotype; HIV infection; HIV Infections - complications; HIV patients; HPV infection; Humans; Identification and classification; INFECTIOUS DISEASES; Middle Aged; Original; Papillomaviridae - genetics; Papillomavirus infections; Papillomavirus Infections - complications; Papillomavirus Infections - virology; Physiological aspects; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Socioeconomic Factors; Brazil; HPV infection; HIV infection; Epidemiology; Cervical cancer
• ISSN: 1413-8670; 1678-4391; 1678-4391
• DOI: 10.1016/j.bjid.2014.07.005

It is believed that Human Papillomavirus (HPV) and Human Immunodeficiency Virus coinfection contributes to increase the risk for cervical intraepithelial injuries. Several factors may contribute to cervical cancer (CC) development, including genetic variants such as TP53 and MDM2 gene polymorphisms.
A hundred HIV-infected women were examined for HPV detection and its genotypes, as well as the frequencies of the SNPs Arg72Pro and SNP309 and their associations with CC risk factors. Nested Polymerase Chain Reaction (nPCR) was used for HPV detection and PCR-RFLP for TP53 and MDM2 SNP309 genotyping.
HPV DNA was detected in 68% of samples. A higher frequency of low-risk HPV genotypes (66.7%) was observed when compared to high-risk genotypes (33.3%). Nine different HPV genotypes were identified, with the highest prevalence of HPV-6, followed by HPV-16 and 31. p53 Arg72Arg and SNP309 TG genotype were the most prevalent. HPV genotyping was performed by sequencing.
The data obtained suggest that HIV-infected women are more susceptible to be infected by low-risk HPV (LR-HPV) genotypes than by high-risk (HR-HPV), and Pro72Pro of TP53 gene and TG of MDM2 SNP309 genotypes apparently seem to be protective factors among HIV-infected women for HPV acquisition and HR-HPV infection, respectively, in a sample of Southern Brazilian woman. Future investigations in larger populations are necessary to better understand the potential roles of these SNPs and the behavior of non-oncogenic HPV genotypes in HIV-mediated immunosuppression cases.