Expression of TAR RNA-Binding Protein in Baculovirus and Co-Immunoprecipitation with Insect Cell Protein Kinase

• Published in: Journal of biomedical science Vol. 2; no. 4; pp. 322 - 329
• Main Authors: Blair, Edward D., Roberts, Christopher M., Snowden, Wendy B., Gatignol, Anne, Benkirange, Monsef, Jeang, Kuan-Teh
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland 01-04-1995
• Subjects: Original Paper; HIV-1; RNA-binding protein; TAR RNA; TRBP; Tat; PKR
• ISSN: 1021-7770; 1423-0127
• DOI: 10.1159/000456872

TAR RNA-binding protein TRBP was originally isolated by its binding affinity for radiolabeled HIV-1 leader RNA. Subsequent studies have suggested that this protein is one member of a family of double-stranded RNA-binding proteins. Recent findings indicate that TRBP might function to antagonize the translational inhibitory effect that can be mediated through cellular protein kinase, PKR. Here, we report on the over-expression of a cDNA coding for TRBP in eukaryotic SF9 cells using baculovirus. We characterized the nuclear localization of TRBP in insect cells, and we demonstrate that TRBP co-immu- noprecipitates with a protein in these cells antigenically related to human PKR.