A PINOID-Dependent Binary Switch in Apical-Basal PIN Polar Targeting Directs Auxin Efflux

• Published in: Science (American Association for the Advancement of Science) Vol. 306; no. 5697; pp. 862 - 865
• Main Authors: Friml, Jiří, Yang, Xiong, Michniewicz, Marta, Weijers, Dolf, Quint, Ab, Tietz, Olaf, Benjamins, René, Pieter B. F. Ouwerkerk, Ljung, Karin, Sandberg, Göran, Paul J. J. Hooykaas, Palme, Klaus, Offringa, Remko
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 29-10-2004; The American Association for the Advancement of Science
• Subjects: Arabidopsis; Arabidopsis - cytology; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Auxins; Biological and medical sciences; Biological Transport; Embryos; Epidermal cells; Flowers & plants; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genetic aspects; Growth regulators; Hormones; Individualized Instruction; Indoleacetic Acids - metabolism; Inflorescences; Logical Thinking; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Meristem - metabolism; Metabolism; Mutation; Perceptual localization; Physiological aspects; Physiological regulation; Pituitary gland; Plant Epidermis - cytology; Plant Epidermis - metabolism; Plant hormones; Plant physiology and development; Plant Roots - metabolism; Plant Shoots - metabolism; Plants, Genetically Modified; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Proteins; Recombinant Fusion Proteins - metabolism; Root meristems; Root tips; Seedlings; Seeds; Seeds - metabolism; Translocation; Auxin; Enzyme; Transferases; Inflorescence; Genetic determinism; Organogenesis; Arabidopsis thaliana; Cruciferae; Protein kinase; Dicotyledones; Angiospermae; Polarity; Spermatophyta; Mutation; Biological accumulation
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1100618

Polar transport-dependent local accumulation of auxin provides positional cues for multiple plant patterning processes. This directional auxin flow depends on the polar subcellular localization of the PIN auxin efflux regulators. Overexpression of the PINOID protein kinase induces a basal-to-apical shift in PIN localization, resulting in the loss of auxin gradients and strong defects in embryo and seedling roots. Conversely, pid loss of function induces an apical-to-basal shift in PIN1 polar targeting at the inflorescence apex, accompanied by defective organogenesis. Our results show that a PINOID-dependent binary switch controls PIN polarity and mediates changes in auxin flow to create local gradients for patterning processes.