Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity

• Published in: Journal of Zhejiang University. Science Vol. 5; no. 4; pp. 472 - 476
• Main Author: 韩明勇 郑树 于金明 彭佳萍 郭其森 王家林
• Format: Journal Article
• Language: English
• Published: China 01-04-2004
• Subjects: Animals; Breast Neoplasms - genetics; Breast Neoplasms - immunology; Breast Neoplasms - therapy; Cancer Vaccines - genetics; Cancer Vaccines - immunology; Cell Line, Tumor; Female; Gene Expression; Gene Transfer Techniques; Humans; Interleukin-18 - genetics; Mice; Mice, Nude; Neoplasm Transplantation; Recombinant Proteins - genetics; RNA, Messenger - genetics; Transfection; Transplantation, Heterologous; 基因转移; 白细胞间介素; 肿瘤发生学
• ISSN: 1009-3095

To study the effect of interleukin-18 gene transfection on the tumorigenesis of breast cancer cell line Bacp37,human breast cancer cell line Bcap37 were transfected with Lipofectamine and selected by G418.The biological expression of rhIL-18 was tested by RT-PCR and ELISA method;nude mice were injected with Bcap37 cell with or without the hIL-18 gene.The hIL-18 cDNA was successfully integrated into Bcap37 cell; 126.3±4.5pg hIL-18 secreted by one million transduced cells in 24 hours. Nude mice injected with IL-18 gene engineered Bcap37 cell had no tumor growth.These findings indicated that human breast cancer cells were successfully modified by the gene of IL-18 cytokine;the IL-18 gene engineered Bcap37 cells secreted hIL-18 and lost their tumorigenicity.The Bcap37 cells transduced with IL-18 gene may be used as breast cancer vaccine.