Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans

There is considerable insight into pathways and genes associated with heat-stress conditions. Most genes involved in stress response have been identified using mutant screens or gene knockdowns. Yet, there is limited understanding of the temporal dynamics of global gene expression in stressful envir...

Full description

Saved in:

Bibliographic Details
Published in:	PloS one Vol. 12; no. 12; p. e0189445
Main Authors:	Jovic, Katharina, Sterken, Mark G, Grilli, Jacopo, Bevers, Roel P J, Rodriguez, Miriam, Riksen, Joost A G, Allesina, Stefano, Kammenga, Jan E, Snoek, L Basten
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 11-12-2017 Public Library of Science (PLoS)
Subjects:	Aging Bioinformatics Biology and Life Sciences Caenorhabditis elegans Damage accumulation EPS Gene expression Genes Genetic aspects Genomes Groep Koornneef Heat Heat stress Heat tolerance Laboratories Laboratorium voor Nematologie Laboratory of Nematology Metabolism Nematodes PE&RC Phenotypes Physical Sciences Progeny Proteins Research and Analysis Methods Screens Stress response Transcription United States > US Illinois Chicago Illinois
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	There is considerable insight into pathways and genes associated with heat-stress conditions. Most genes involved in stress response have been identified using mutant screens or gene knockdowns. Yet, there is limited understanding of the temporal dynamics of global gene expression in stressful environments. Here, we studied global gene expression profiles during 12 hours of heat stress in the nematode C. elegans. Using a high-resolution time series of increasing stress exposures, we found a distinct shift in gene expression patterns between 3-4 hours into the stress response, separating an initially highly dynamic phase from a later relatively stagnant phase. This turning point in expression dynamics coincided with a phenotypic turning point, as shown by a strong decrease in movement, survival and, progeny count in the days following the stress. Both detectable at transcriptional and phenotypic level, this study pin-points a relatively small time frame during heat stress at which enough damage is accumulated, making it impossible to recover the next few days.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0189445