Myo1e impairment results in actin reorganization, podocyte dysfunction, and proteinuria in zebrafish and cultured podocytes

• Published in: PloS one Vol. 8; no. 8; p. e72750
• Main Authors: Mao, Jianhua, Wang, Dayan, Mataleena, Parikka, He, Bing, Niu, Dadi, Katayama, Kan, Xu, Xiangjun, Ojala, Juha Rm, Wang, Wenjing, Shu, Qiang, Du, Lizhong, Liu, Aimin, Pikkarainen, Timo, Patrakka, Jaakko, Tryggvason, Karl
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19-08-2013; Public Library of Science (PLoS)
• Subjects: Actin; Actins - metabolism; Animals; Biochemistry; Biology; Biophysics; Cell Count; Cell migration; Cell Migration Assays; Cell Proliferation; Cell Shape; Cell size; Cells, Cultured; Cysts; Cytoskeleton; Cytoskeleton - metabolism; Danio rerio; DNA polymerases; Down-Regulation; Edema; Endocytosis; Fertilization; Filtration; Fluorescein-5-isothiocyanate - metabolism; Gene Knockdown Techniques; Hospitals; Humans; Immunofluorescence; Impairment; Kidney Diseases - metabolism; Kidney Diseases - pathology; Kidney Glomerulus - metabolism; Kidneys; Kinases; Larvae; Medicin och hälsovetenskap; Medicine; Mice; Muscle proteins; Myosin Type I - metabolism; Myosins - metabolism; Nephrology; Podocytes - metabolism; Proteins; Proteinuria; Proteinuria - metabolism; RNA; RNA-directed DNA polymerase; Rodents; Transferrin - metabolism; Zebrafish; Zebrafish - metabolism; Zebrafish Proteins - metabolism; Sweden; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0072750

Podocytes serve as an important constituent of the glomerular filtration barrier. Recently, we and others identified Myo1e as a key molecular component of the podocyte cytoskeleton.
Myo1e mRNA and protein was expressed in human and mouse kidney sections as determined by Northern blot and reverse transcriptase PCR, and its expression was more evident in podocytes by immunofluorescence. By specific knock-down of MYO1E in zebrafish, the injected larvae exhibited pericardial edema and pronephric cysts, consistent with the appearance of protein in condensed incubation supernate. Furthermore, specific inhibition of Myo1e expression in a conditionally immortalized podocyte cell line induced morphological changes, actin cytoskeleton rearrangement, and dysfunction in cell proliferation, migration, endocytosis, and adhesion with the glomerular basement membrane.
Our results revealed that Myo1e is a key component contributing to the functional integrity of podocytes. Its impairment may cause actin cytoskeleton re-organization, alteration of cell shape, and membrane transport, and podocyte drop-out from the glomerular basement membrane, which might eventually lead to an impaired glomerular filtration barrier and proteinuria.