Transfer of Maternal Immune Cells by Breastfeeding: Maternal Cytotoxic T Lymphocytes Present in Breast Milk Localize in the Peyer's Patches of the Nursed Infant

• Published in: PloS one Vol. 11; no. 6; p. e0156762
• Main Authors: Cabinian, Allison, Sinsimer, Daniel, Tang, May, Zumba, Osvaldo, Mehta, Hetali, Toma, Annmarie, Sant'Angelo, Derek, Laouar, Yasmina, Laouar, Amale
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10-06-2016; Public Library of Science (PLoS)
• Subjects: Adaptive systems; Animals; Animals, Newborn; Animals, Suckling; Antibodies; Biology and Life Sciences; Breast; Breast feeding; Breast milk; Breastfeeding & lactation; CCR9 protein; CD8 antigen; Cell activation; Cell survival; Cells, Cultured; Chemotaxis, Leukocyte - physiology; Cytotoxicity; Female; Health aspects; Health risks; Homing; Immune system; Immunity; Immunity, Maternally-Acquired - immunology; Immunization, Passive - methods; Immunology; Infants; Inflammation; Intestine; L-selectin; Lactation - immunology; Leukocytes; Lymphocytes; Lymphocytes T; Major histocompatibility complex; Medicine and Health Sciences; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Milk; Milk - cytology; Milk - immunology; Mothers; Myeloid cells; Nursing; Patches (structures); People and Places; Peyer's patches; Peyer's Patches - cytology; Peyer's Patches - immunology; Suckling behavior; T-Lymphocytes, Cytotoxic - cytology; T-Lymphocytes, Cytotoxic - physiology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0156762

Despite our knowledge of the protective role of antibodies passed to infants through breast milk, our understanding of immunity transfer via maternal leukocytes is still limited. To emulate the immunological interface between the mother and her infant while breast-feeding, we used murine pups fostered after birth onto MHC-matched and MHC-mismatched dams. Overall, data revealed that: 1) Survival of breast milk leukocytes in suckling infants is possible, but not significant after the foster-nursing ceases; 2) Most breast milk lymphocytes establish themselves in specific areas of the intestine termed Peyer's patches (PPs); 3) While most leukocytes in the milk bolus were myeloid cells, the majority of breast milk leukocytes localized to PPs were T lymphocytes, and cytotoxic T cells (CTLs) in particular; 4) These CTLs exhibit high levels of the gut-homing molecules α4β7 and CCR9, but a reduced expression of the systemic homing marker CD62L; 5) Under the same activation conditions, transferred CD8 T cells through breast milk have a superior capacity to produce potent cytolytic and inflammatory mediators when compared to those generated by the breastfed infant. It is therefore possible that maternal CTLs found in breast milk are directed to the PPs to compensate for the immature adaptive immune system of the infant in order to protect it against constant oral infectious risks during the postnatal phase.