ARF6 promotes the formation of Rac1 and WAVE-dependent ventral F-actin rosettes in breast cancer cells in response to epidermal growth factor

• Published in: PloS one Vol. 10; no. 3; p. e0121747
• Main Authors: Marchesin, Valentina, Montagnac, Guillaume, Chavrier, Philippe
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23-03-2015; Public Library of Science (PLoS)
• Subjects: Actin; Actin Cytoskeleton - drug effects; Actin Cytoskeleton - metabolism; Actins - metabolism; Activation; ADP-Ribosylation Factors - deficiency; ADP-Ribosylation Factors - genetics; ADP-Ribosylation Factors - metabolism; Biology; Breast cancer; Breast Neoplasms - pathology; Cancer; Cell adhesion & migration; Cell Line, Tumor; Cell Membrane - drug effects; Cell Membrane - metabolism; Cell migration; Cell Movement - drug effects; Cell surface; Cytoskeleton; Epidermal growth factor; Epidermal Growth Factor - pharmacology; Epidermal growth factors; Gene Silencing; Humans; Immunoglobulins; Invasiveness; Kinases; Medical prognosis; Motility; Muscle proteins; Phenotypes; Plasma; Polymerization; Proteins; rac1 GTP-Binding Protein - metabolism; Rac1 protein; Receptor, Epidermal Growth Factor - metabolism; Receptors; RNA, Small Interfering - genetics; Signal Transduction - drug effects; Signaling; Tumors; Up-Regulation - drug effects; Wiskott-Aldrich Syndrome Protein Family - metabolism; France; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0121747

Coordination between actin cytoskeleton assembly and localized polarization of intracellular trafficking routes is crucial for cancer cell migration. ARF6 has been implicated in the endocytic recycling of surface receptors and membrane components and in actin cytoskeleton remodeling. Here we show that overexpression of an ARF6 fast-cycling mutant in MDA-MB-231 breast cancer-derived cells to mimick ARF6 hyperactivation observed in invasive breast tumors induced a striking rearrangement of the actin cytoskeleton at the ventral cell surface. This phenotype consisted in the formation of dynamic actin-based podosome rosette-like structures expanding outward as wave positive for F-actin and actin cytoskeleton regulatory components including cortactin, Arp2/3 and SCAR/WAVE complexes and upstream Rac1 regulator. Ventral rosette-like structures were similarly induced in MDA-MB-231 cells in response to epidermal growth factor (EGF) stimulation and to Rac1 hyperactivation. In addition, interference with ARF6 expression attenuated activation and plasma membrane targeting of Rac1 in response to EGF treatment. Our data suggest a role for ARF6 in linking EGF-receptor signaling to Rac1 recruitment and activation at the plasma membrane to promote breast cancer cell directed migration.