Secretome Profiling of Periodontal Ligament from Deciduous and Permanent Teeth Reveals a Distinct Expression Pattern of Laminin Chains

• Published in: PloS one Vol. 11; no. 5; p. e0154957
• Main Authors: Giovani, Priscila A, Salmon, Cristiane R, Martins, Luciane, Paes Leme, Adriana F, Rebouças, Pedro, Puppin Rontani, Regina M, Mofatto, Luciana S, Sallum, Enilson A, Nociti, Jr, Francisco H, Kantovitz, Kamila R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05-05-2016; Public Library of Science (PLoS)
• Subjects: Adult; Bioinformatics; Biology and Life Sciences; Cell adhesion; Cell adhesion molecules; Cell Adhesion Molecules - metabolism; Cells, Cultured; Chains; Child; Comparative analysis; Data analysis; Data processing; Dental schools; Dentistry; Dentition, Permanent; Extracellular matrix; Female; Fibroblasts; Gene expression; Gene Expression Profiling - methods; Genetic aspects; Homeostasis; Humans; Laminin; Laminin - metabolism; Ligaments; Male; Medicine and Health Sciences; Orthodontics; Pediatrics; Periodontal ligament; Periodontal Ligament - metabolism; Physiological aspects; Physiology; Protein expression; Proteins; Proteomics; Research and Analysis Methods; Ribonucleic acid; RNA; Secretome; Teeth; Tissues; Tooth, Deciduous - metabolism; Western blotting; Young Adult; Brazil
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0154957

It has been suggested that there are histological and functional distinctions between the periodontal ligament (PDL) of deciduous (DecPDL) and permanent (PermPDL) teeth. Thus, we hypothesized that DecPDL and PermPDL display differences in the constitutive expression of genes/proteins involved with PDL homeostasis. Primary PDL cell cultures were obtained for DecPDL (n = 3) and PermPDL (n = 3) to allow us to perform label-free quantitative secretome analysis. Although a highly similar profile was found between DecPDL and PermPDL cells, comparative secretome analysis evidenced that one of the most stickling differences involved cell adhesion molecules, including laminin subunit gamma 1 (LAMC1) and beta 2 (LAMB2). Next, total RNA and protein extracts were obtained from fresh PDL tissues of deciduous (n = 6) and permanent (n = 6) teeth, and Western blotting and qPCR analysis were used to validate our in vitro findings. Western blot analysis confirmed that LAMC1 was increased in DecPDL fresh tissues (p<0.05). Furthermore, qPCR data analysis revealed that mRNA levels for laminin subunit beta 1 (LAMB1), beta 3 (LAMB3), LAMC1, and gamma 2 (LAMC2) were higher in DecPDL fresh tissues, whereas transcripts for LAMB2 were increased in PermPDL (p<0.05). In conclusion, the differential expression of laminin chains in DecPDL and PermPDL suggests an involvement of laminin-dependent pathways in the control of physiological differences between them.