Characteristics of bile acid composition in high fat diet-induced nonalcoholic fatty liver disease in obese diabetic rats

• Published in: PloS one Vol. 16; no. 2; p. e0247303
• Main Authors: Nakade, Yukiomi, Kitano, Rena, Sakamoto, Kazumasa, Kimoto, Satoshi, Yamauchi, Taeko, Inoue, Tadahisa, Kobayashi, Yuji, Ohashi, Tomohiko, Sumida, Yoshio, Ito, Kiyoaki, Yoneda, Masashi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 24-02-2021; Public Library of Science (PLoS)
• Subjects: Acids; Adipose tissue; Animals; Bile; Bile acids; Bile Acids and Salts - metabolism; Biology and Life Sciences; Blood Glucose - metabolism; Cholic acid; Coenzyme A; Composition; Conjugation; Deoxycholic acid; Development and progression; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental; Diet; Diet, High-Fat; Energy metabolism; Fatty Acids - metabolism; Fatty liver; Funding; Gastroenterology; Gene expression; Gene Expression Regulation; Glucagon-Like Peptide 1 - blood; High fat diet; Insulin; Insulin Resistance; Liver; Liver - metabolism; Liver diseases; Male; Medicine; Medicine and Health Sciences; Metabolism; Muscles; Non-alcoholic Fatty Liver Disease - blood; Non-alcoholic Fatty Liver Disease - complications; Non-alcoholic Fatty Liver Disease - genetics; Non-alcoholic Fatty Liver Disease - metabolism; Obesity; Obesity - blood; Obesity - complications; Obesity - genetics; Obesity - metabolism; Polypeptides; Protein turnover; Proteins; Rats; Rats, Sprague-Dawley; Receptors; Regulatory sequences; Triglycerides; Triglycerides - metabolism; Japan; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0247303

Bile acid has attracted attention as a signal transmission molecule in energy metabolism. Although a high-fat diet (HFD) or obesity is known to increase hepatic fat content and alter bile acid composition, the changes in bile acid composition due to HFD or obesity remain to be elucidated. We sought to examine the bile acid composition in high fat diet-induced non-alcoholic fatty liver disease (NAFLD) in obese diabetic rats. Eight-week-old male spontaneously diabetic Torii fatty (SDTF) rats or control rats were fed an HFD. Twelve weeks post the commencement of HFD, serum and hepatic bile acid compositions and serum GLP-1 levels, which is stimulated by the secondary bile acid deoxycholic acid (DCA), were measured. The correlation between the bile acid composition and serum GLP-1 levels was also examined. While serum and hepatic levels of cholic acid (CA), a primary bile acid, tended to decrease in HFD-fed control rats, they were significantly decreased in HFD-fed SDTF rats. Hepatic CYP8B1, which plays a role in CA synthesis, the mRNA levels were significantly decreased in HFD-fed control and SDTF rats. In contrast, while serum and hepatic DCA levels were not changed in HFD-fed control rats, they were decreased in HFD-fed SDTF rats. Hepatic DCA/CA did not change in HFD-fed SDTF rats, but significantly increased in HFD-fed control rats. While serum GLP-1 levels were not changed in SDTF rats, they were significantly increased in HFD-fed control rats. Hepatic DCA/CA tended to correlate with serum GLP-1 levels, which tended to negatively correlate with the hepatic triglyceride content in SDTF rats. These results indicate that relatively increased DCA might contribute to an increase in serum GLP-1 levels, which inhibits hepatic steatosis in NAFLD.