Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla

• Published in: PloS one Vol. 14; no. 6; p. e0217627
• Main Authors: Kaaniche, Fatma, Hamed, Abdelaaty, Abdel-Razek, Ahmed S, Wibberg, Daniel, Abdissa, Negera, El Euch, Imene Zendah, Allouche, Noureddine, Mellouli, Lotfi, Shaaban, Mohamed, Sewald, Nobert
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27-06-2019; Public Library of Science (PLoS)
• Subjects: Acetylcholinesterase; Agricultural production; Antioxidants; Antioxidants (Nutrients); Antioxidants - pharmacology; Ascomycota - classification; Ascomycota - isolation & purification; Ascomycota - metabolism; Bacteria - drug effects; Base sequence; Bioactive compounds; Biological activity; Biology and Life Sciences; Biotechnology; Chemical compounds; Cholinesterase Inhibitors - pharmacology; Chromatography; Deoxyribonucleic acid; Diagnostic imaging; DNA; Endophytes; Endophytes - metabolism; Ergosterol; Fermentation; Free Radical Scavengers - pharmacology; Fungi; Herbal medicine; Homology; Host plants; Laboratories; Magnetic resonance spectroscopy; Mass spectrometry; Mass spectroscopy; Medicinal plants; Metabolites; Microbial Sensitivity Tests; Microorganisms; Natural products; NMR; NMR spectroscopy; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Nucleotide sequence; Nucleotides; Organic chemistry; Pharmaceutical sciences; Pharmacology; Phylogeny; Phytochemicals - chemistry; Phytochemicals - pharmacology; Phytosterols; Plant lipids; Plant metabolites; Rauwolfia - microbiology; Research and analysis methods; Ribosomal DNA; Secondary Metabolism; Secondary metabolites; Spacer; Spectroscopy; Nigeria; Cameroon; Cairo Egypt; Tunisia; Germany; Egypt
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0217627

Over the last decades, endophytic fungi represent a new source of pharmacologically active secondary metabolites based on the underlying assumption that they live symbiotically within their plant host. In the present study, a new endophytic fungus was isolated from Rauwolfia macrophylla, a medicinal plant from Cameroon. The fungus showed a highest homology to Curvularia sp. based on complete nucleotide sequence data generated from the internal transcribed spacer (ITS) of ribosomal DNA region. Large scale fermentation, working-up and separation of the strain extract using different chromatographic techniques afforded three bioactive compounds: 2'-deoxyribolactone (1), hexylitaconic acid (2) and ergosterol (3). The chemical structures of compounds 1-3 were confirmed by 1 and 2D NMR spectroscopy and mass spectrometry, and comparison with corresponding literature data. Biologically, the antimicrobial, antioxidant activities and the acetylcholinesterase inhibitory of the isolated compounds were studied.