Identification of phenotypic markers of B cells from patients with C hagas disease
Chagas disease was discovered more than a hundred years ago, but its pathogenesis is still not completely understood. Autoimmunity is one of the mechanisms shown to contribute to its pathogenesis, which may indicate an important participation of B lymphocytes. Patients with C hagas disease have show...
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Published in: | Parasite immunology Vol. 35; no. 7-8; pp. 214 - 223 |
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Main Authors: | , , , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
01-07-2013
|
Online Access: | Get full text |
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Summary: | Chagas disease was discovered more than a hundred years ago, but its pathogenesis is still not completely understood. Autoimmunity is one of the mechanisms shown to contribute to its pathogenesis, which may indicate an important participation of
B
lymphocytes. Patients with
C
hagas disease have shown increased percentage of
B
cells producing
IL
‐10. However, there are no reports of the phenotypic markers of
B
cells producing
IL
‐10 in patients with
C
hagas disease. For the first time in the literature, we evaluated the phenotypic profile of distinct markers of
B
cells from peripheral blood of noninfected individuals and patients with
C
hagas disease. Our results showed that patients with
C
hagas disease had a higher expression of
CD
21 and
CD
24 on the surface of
CD
19
+
B
cells, while
CD
43 and
CD
23 were expressed equally in all groups. Moreover, the expression of
MHC
‐
II
(
HLA
‐
DR
),
CD
80,
CD
86, caspase‐3, granzyme
B
and intracellular
IL
‐10 and
TGF
‐β by
CD
19
+
B
cells was higher in patients with
C
hagas disease. The results of
IL
‐10 production within
CD
19
+
CD
5
+
CD
1d
+
B
cells showed a higher percentage of this cytokine in patients with
C
hagas disease. Thus, our data bring a new knowledge about distinct markers of
B
cells in immune responses of
C
hagas disease. |
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ISSN: | 0141-9838 1365-3024 |
DOI: | 10.1111/pim.12038 |