tappAS: a comprehensive computational framework for the analysis of the functional impact of differential splicing

• Published in: Genome Biology Vol. 21; no. 1; pp. 119 - 32
• Main Authors: de la Fuente, Lorena, Arzalluz-Luque, Ángeles, Tardáguila, Manuel, Del Risco, Héctor, Martí, Cristina, Tarazona, Sonia, Salguero, Pedro, Scott, Raymond, Lerma, Alberto, Alastrue-Agudo, Ana, Bonilla, Pablo, Newman, Jeremy R B, Kosugi, Shunichi, McIntyre, Lauren M, Moreno-Manzano, Victoria, Conesa, Ana
• Format: Journal Article
• Language: English
• Published: England BioMed Central 18-05-2020; BMC
• Subjects: Alternative Splicing; Animals; Annotations; Biology; Computer applications; Gene expression; Gene Expression Profiling - methods; Gene regulation; Java; Mice; Oligodendrocyte Precursor Cells - metabolism; Polyadenylation; Post-transcription; Protein Isoforms - metabolism; Proteins; Researchers; Ribonucleic acid; RNA; Software; Splicing; Studies; Transcriptomics; User interface
• ISSN: 1474-760X; 1474-7596; 1474-760X
• DOI: 10.1186/s13059-020-02028-w

Recent advances in long-read sequencing solve inaccuracies in alternative transcript identification of full-length transcripts in short-read RNA-Seq data, which encourages the development of methods for isoform-centered functional analysis. Here, we present tappAS, the first framework to enable a comprehensive Functional Iso-Transcriptomics (FIT) analysis, which is effective at revealing the functional impact of context-specific post-transcriptional regulation. tappAS uses isoform-resolved annotation of coding and non-coding functional domains, motifs, and sites, in combination with novel analysis methods to interrogate different aspects of the functional readout of transcript variants and isoform regulation. tappAS software and documentation are available at https://app.tappas.org.