Urinary 1-hydroxypyrene and 8-hydroxydeoxyguanosine Levels among Coke-oven Workers for 2 Consecutive Days

• Published in: Journal of Occupational Health Vol. 56; no. 3; pp. 178 - 185
• Main Authors: Nguyen, Thi‐To‐Uyen, Kawanami, Shoko, Kawai, Kazuaki, Kasai, Hiroshi, Li, Yun‐Shan, Inoue, Jinro, Ngoan, Le Tran, Horie, Seichi
• Format: Journal Article
• Language: English
• Published: Australia JAPAN SOCIETY FOR OCCUPATIONAL HEALTH 01-05-2014; John Wiley & Sons, Inc
• Subjects: 1‐Hydroxypyrene; 8‐Hydroxydeoxyguanosine; Adult; Air Pollutants, Occupational - analysis; Case-Control Studies; Coke; Coke oven; Correlation; Creatinine; Damage; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - toxicity; Deoxyguanosine - urine; Deoxyribonucleic acid; DNA; DNA Damage; Exposure; Female; Humans; Industry; Liquid chromatography; Male; Middle Aged; Occupational exposure; Occupational Exposure - analysis; Occupational health; Oxidative Stress; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - toxicity; Pyrenes - toxicity; Pyrenes - urine; Regression analysis; Smoking; Statistical analysis; Urine; Vietnam
• ISSN: 1341-9145; 1348-9585; 1348-9585
• DOI: 10.1539/joh.13-0222-OA

[Abstract]: [Objectives]: This study evaluated the levels of exposure to polycyclic aromatic hydrocarbons (PAHs) and their relationship with oxidative DNA damage among Vietnamese coke-oven workers. [Methods]: We collected urine from 36 coke-oven workers (exposed group) at the beginning and end of the shift on 2 consecutive days. We also collected urine from 78 medical staff (control group). Information was collected by questionnaire about smoking status, drinking habit, and working position. Urinary 1-hydroxypyrene (1-OHP) and 8-hydroxydeoxyguanosine (8-OH-dG) were measured using HPLC. All statistical analyses were performed with SPSS version 19. [Results]: Urinary 1-OHP was significantly higher in the coke-oven workers than in the control group (p<0.05). Top-oven workers had the highest levels of internal exposure to PAHs, followed by side-oven and then bottom-oven workers (5.41, 4.41 and 1.35 ng/mg creatinine, respectively, at the end of the shift on day 2). Urinary 8-OH-dG was significantly higher in top- and side-oven workers at the end of the shift on day 2 (4.63 and 5.88 ng/mg creatinine, respectively) than in the control group (3.85 ng/mg creatinine). Based on a multi-regression analysis, smoking status had a significant effect on urinary 8-OH-dG (p=0.049). Urinary 1-OHP tended to have a positive correlation with urinary 8-OH-dG (p=0.070). [Conclusions]: Vietnamese coke-oven workers were exposed to PAHs during their work shift. Urinary 1-OHP exceeded the recommended limit, and elevated oxidative DNA damage occurred in top- and side-oven workers on the second day of work. A tendency for positive correlation was found between urinary 1-OHP and urinary 8-OH-dG.