In vitro susceptibility testing of amphotericin B for Cryptococcus neoformans variety grubii AFLP 1/ VN I and Cryptococcus gattii AFLP 6/ VG II by CLSI and flow cytometry

• Published in: Mycoses Vol. 58; no. 5; pp. 273 - 279
• Main Authors: Morales, Bernardina Penarrieta, Trilles, Luciana, Bertho, Álvaro Luiz, Junior, Ivan Neves, de Oliveira, Raquel de Vasconcellos Carvalhaes, Wanke, Bodo, Lazéra, Márcia dos Santos
• Format: Journal Article
• Language: English
• Published: 01-05-2015
• ISSN: 0933-7407; 1439-0507
• DOI: 10.1111/myc.12310

Summary
Cryptococcus neoformans
var.
grubii
AFLP
1/
VNI
is the main causative agent of cryptococcosis associated with
AIDS
in the world.
Cryptococcus gattii
AFLP
6/
VGII
causes mainly endemic primary infection in immunocompetent hosts. To determine the minimum inhibitory concentrations (
MIC
s) of
C. neoformans
var.
grubii
AFLP
1/
VNI
and
C. gattii
AFLP
6/
VGII
against amphotericin B (
AMB
) in a short period of time, flow cytometry (
FCM
) with
FUN
‐1 fluorochrome was used to compare with broth microdilution method (
CLSI
M27‐A3). The minimum incubation period was evaluated by minimum fungicidal concentration procedure. Seventeen clinical isolates of
C. neoformans
var.
grubii
AFLP
1/
VNI
and 18 of
C. gattii
AFLP
6/
VGII
were analysed. The time for the determination of
MIC
s by
FCM
was 2 h against 72 h by
CLSI
M27‐A3 and the comparison of
MIC
showed a positive significant correlation (
P 
= 0.048). It is important to highlight the role of the
FCM
as an alternative method to determine the
MIC
s for
AMB
in within a day, with positive cost‐benefit.