Application of transcutaneous carbon dioxide improves capillary regression of skeletal muscle in hyperglycemia

• Published in: The journal of physiological sciences Vol. 69; no. 2; pp. 317 - 326
• Main Authors: Matsumoto, Tomohiro, Tanaka, Masayuki, Ikeji, Takuya, Maeshige, Noriaki, Sakai, Yoshitada, Akisue, Toshihiro, Kondo, Hiroyo, Ishihara, Akihiko, Fujino, Hidemi
• Format: Journal Article
• Language: English
• Published: Japan Springer 01-03-2019; BioMed Central; Springer Japan
• Subjects: Angiogenesis; Animals; Blood flow; Blood Glucose - drug effects; Capillaries - drug effects; Capillaries - metabolism; Carbon dioxide; Carbon Dioxide - administration & dosage; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Hyperglycemia; Hyperglycemia - drug therapy; Hyperglycemia - metabolism; Male; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscles; Nitric Oxide Synthase Type III - metabolism; Original Paper; Rats; Rats, Wistar; Regional Blood Flow - drug effects; Rodents; Skeletal muscle; Soleus muscle; Streptozocin; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism; Japan; CO2 therapy; Muscle capillary; Pro-angiogenesis factors; Diabetes; Anti-angiogenesis factors; Blood flow
• ISSN: 1880-6546; 1880-6562
• DOI: 10.1007/s12576-018-0648-y

The purpose of the present study was to determine the effects of transcutaneous CO
application on the blood flow and capillary architecture of the soleus muscle in rats with streptozotocin (STZ)-induced hyperglycemia. Wistar rats were randomly divided into four groups: control, control + CO
-treated, STZ-induced hyperglycemia, and STZ-induced hyperglycemia + CO
-treated groups. Blood flow in soleus muscle increased during the transcutaneous CO
exposure, and continued to increase for 30 min after the treatment. In addition, the transcutaneous CO
attenuated a decrease in capillary and the expression level of eNOS and VEGF protein, and an increase in the expression level of MDM-2 and TSP-1 protein of soleus muscle due to STZ-induced hyperglycemia. These results indicate that the application of transcutaneous CO
could improve capillary regression via the change of pro- and anti-angiogenesis factors, which might be induced by an increase in blood flow.