Peptide and Protein Synthesis by Segment Synthesis-Condensation

• Published in: Science (American Association for the Advancement of Science) Vol. 243; no. 4888; pp. 187 - 192
• Main Authors: Kaiser, Emil Thomas, Mihara, Hisakazu, Laforet, Genevieve A., Kelly, Jeffery W., Walters, Lee, Findeis, Mark A., Sasaki, Tomikazu
• Format: Journal Article
• Language: English
• Published: Washington, DC The American Association for the Advancement of Science 13-01-1989; American Association for the Advancement of Science
• Subjects: Amino Acid Sequence; Amino acid sequencing; Amino acids; Analysis; Apolipoprotein A-I; Apolipoproteins; Apolipoproteins A - chemical synthesis; Biochemistry; Chemical synthesis; Chemistry; Condensation; DNA; Enzymes; Exact sciences and technology; Humans; Indicators and Reagents; Lipoproteins, HDL - chemical synthesis; Mass spectroscopy; Molecules; Organic chemistry; Organic compound synthesis; Organic compounds; Oximes; Peptide synthesis; Peptides; Peptides - chemical synthesis; Preparations and properties; Protein Conformation; Proteins; Proteins - chemical synthesis; Resins; Synthesis; Synthetic products; Proteins; Liquid phase; Solid phase; Oligopeptides; Enzyme
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.2492114

The chemical synthesis of biologically active peptides and polypeptides can be achieved by using a convergent strategy of condensing protected peptide segments to form the desired molecule. An oxime support increases the ease with which intermediate protected peptides can be synthesized and makes this approach useful for the synthesis of peptides in which secondary structural elements have been redesigned. The extension of these methods to large peptides and proteins, for which folding of secondary structures into functional tertiary structures is critical, is discussed. Models of apolipoproteins, the homeo domain from the developmental protein encoded by the Antennapedia gene of Drosophila, a part of the Cro repressor, and the enzyme ribonuclease T$_{1}$ and a structural analog have been synthesized with this method.