Evolutionary and Ecological Characterization of Mayaro Virus Strains Isolated during an Outbreak, Venezuela, 2010

• Published in: Emerging infectious diseases Vol. 21; no. 10; pp. 1742 - 1750
• Main Authors: Auguste, Albert J, Liria, Jonathan, Forrester, Naomi L, Giambalvo, Dileyvic, Moncada, Maria, Long, Kanya C, Morón, Dulce, de Manzione, Nuris, Tesh, Robert B, Halsey, Eric S, Kochel, Tadeusz J, Hernandez, Rosa, Navarro, Juan-Carlos, Weaver, Scott C
• Format: Journal Article
• Language: English
• Published: United States U.S. National Center for Infectious Diseases 01-10-2015; Centers for Disease Control and Prevention
• Subjects: alphavirus; Alphavirus - genetics; Alphavirus - growth & development; Alphavirus Infections - epidemiology; Biological Evolution; Biota - immunology; Cladistic analysis; Disease Outbreaks; epidemic; Female; Genomes; Genomics; Humans; Male; Mayaro virus; outbreak; Phylogeny; Venezuela - epidemiology; viruses; Venezuela; South America; Brazil; Trinidad and Tobago; Texas; Venezuela; ecology; viruses; niche modeling; Mayaro virus; coalescent analysis; epidemic; outbreak; phylogeny; zoonoses; alphavirus; evolution
• ISSN: 1080-6040; 1080-6059
• DOI: 10.3201/eid2110.141660

In 2010, an outbreak of febrile illness with arthralgic manifestations was detected at La Estación village, Portuguesa State, Venezuela. The etiologic agent was determined to be Mayaro virus (MAYV), a reemerging South American alphavirus. A total of 77 cases was reported and 19 were confirmed as seropositive. MAYV was isolated from acute-phase serum samples from 6 symptomatic patients. We sequenced 27 complete genomes representing the full spectrum of MAYV genetic diversity, which facilitated detection of a new genotype, designated N. Phylogenetic analysis of genomic sequences indicated that etiologic strains from Venezuela belong to genotype D. Results indicate that MAYV is highly conserved genetically, showing ≈17% nucleotide divergence across all 3 genotypes and 4% among genotype D strains in the most variable genes. Coalescent analyses suggested genotypes D and L diverged ≈150 years ago and genotype diverged N ≈250 years ago. This virus commonly infects persons residing near enzootic transmission foci because of anthropogenic incursions.